We narrowed to 11,735 results for: pcDNA

1. 1a154 (H77)

   Plasmid

   #177689

   Purpose

   HCV E1E2 expression plasmid

   Depositor

   Justin Bailey

   Article

   Salas et al Gastroenterology. 2022 Feb;162(2):562-57

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcpAzpaRS_v2

   Plasmid

   #196483

   Purpose

   To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40(synthetase), Pol III tRNA promoter (tRNA), C…

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTracer_Cr-TRP1

   Plasmid

   #64881

   Purpose

   Expresses CrTRP1 in mammalian cells

   Depositor

   Sebastián Brauchi

   Article

   Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

   Insert

   Transient receptor potential 1
   

   Use

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. BirA-Myc_N_MYC

   Plasmid

   #167715

   Purpose

   Expresses MYC in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. POLArISact

   Plasmid

   #164971

   Purpose

   Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   POLArISact
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Mfn2(K109A)-16xmyc

   Plasmid

   #26051

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Mfn2 (Mfn2 Mouse)
   

   Use

   Tags

   16xMyc

   Expression

   Mammalian

   Mutation

   K109A GTPase mutant

   Promoter

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

8. Flag-V0D1

   Plasmid

   #210348

   Purpose

   Expresses ATP6V0D1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   V-ATPase V0D1 (ATP6V0D1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Dynamin-I-K44A-6His/Flag

   Plasmid

   #32696

   Depositor

   Robert Lefkowitz

   Article

   Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

   Insert

   Dynamin I (Dnm1 Rat)
   

   Use

   Tags

   Flag and His6

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

10. TPI-PTC160

    Plasmid

    #130698

    Purpose

    Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC160 (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    Pcmv

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ALIX(1-702)-mNeonGreen

    Plasmid

    #191193

    Purpose

    Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (1-702) (PDCD6IP Human, Synthetic)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Deletion of ALIX C-terminal PRD.

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FKBP-CD28TMD-NTEVp(H75E)

    Plasmid

    #137829

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75E)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cycLuc_TEVS

    Plasmid

    #119207

    Purpose

    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with TEVs cleavage site
    

    Use

    Luciferase

    Tags

    6xHis and AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. cpGFP-Adhiron_flex

    Plasmid

    #165044

    Purpose

    Flexible linker control for POLArISact expression in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    cpGFP-Adhiron (connected with a flexible linker)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DN-LOXL2

    Plasmid

    #200062

    Purpose

    LOXL2 deletion mutant lacking 1-338 amino acids

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    N-terminus amino acids 1-338 truncated.

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R338G/V339P-LOXL2

    Plasmid

    #200063

    Purpose

    Point mutant of hLOXL2 resistant to processing by Factor Xa

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    S300P mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L1-1180

    Plasmid

    #13265

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated after RSLE region

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

20. GLG1-2Strep

    Plasmid

    #210362

    Purpose

    Expresses GLG1 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    GLG1 (GLG1 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid

    #137830

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Leucine 71 to Lysine in CTEVp fragment

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Diacylglycerol Kinase Zeta

    Plasmid

    #85454

    Purpose

    expression of Diacylglycerol Kinase Zeta

    Depositor

    Stephen Gee

    Article

    Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

    Insert

    Diacylglycerol Kinase Zeta (DGKZ Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAL204

    Plasmid

    #22282

    Purpose

    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-ARP8

    Plasmid

    #15361

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    actin-related protein 8 (ACTR8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CoV2-N-R203K

    Plasmid

    #177951

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203K) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203K

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HA-EGFP-NEMO-D304N

    Plasmid

    #187898

    Purpose

    Mammalian cell expression of D304N mutation in NEMO

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    HA-EGFP-NEMO-D304N (Ikbkg Mouse)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    D304N

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Mfn1(K88T)-10xmyc

    Plasmid

    #26050

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn1 (Mfn1 Mouse)
    

    Use

    Tags

    10xMyc

    Expression

    Mammalian

    Mutation

    K88T GTPase mutant

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

28. CMV-hCas9-T2A-mStrawberry

    Plasmid

    #187224

    Purpose

    Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Inserted T2A-mStrawberry at 3' terminal of M…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. eGFP-SUN1

    Plasmid

    #213515

    Purpose

    Expresses human eGFP-SUN1 in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    SUN1 (SUN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1 has a silent mutation on its gene (nucleotid…

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. dCas9-NLS-gs10-cTRb413

    Plasmid

    #188261

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cTRb413 (TXNRD2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CaV1.3e[8a,11,31b,Δ32,42a] mut

    Plasmid

    #26576

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

32. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. L1dRSLE

    Plasmid

    #13266

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RSLE region removed

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

34. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmPB2-VSV

    Plasmid

    #68038

    Purpose

    Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B2 (Plxnb2 Mouse)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FLAG HDAC5 S259A/S498A

    Plasmid

    #32216

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser259Ala, Ser498Ala

    Promoter

    CMV

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

38. mCherry-Climp63(1–192)

    Plasmid

    #186955

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBCMV-HaloTag-VPg(FCV)

    Plasmid

    #167311

    Purpose

    Template pDNA to prepare a split CaVT component mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    HaloTag-VPg(FCV)
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GA-DEVD-GB-NES

    Plasmid

    #61020

    Purpose

    green ddFP based caspase-3 biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Use

    Tags

    HindIII and NES sequence LALKLAGLDIGS placed afte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

41. Equalizer-H plasmid with onboard mCherry cassette

    Plasmid

    #169733

    Purpose

    Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. V5-bArr1

    Plasmid

    #201491

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    V5-bArr1 (ARRB1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. sq5

    Plasmid

    #24499

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gs alpha (Gnas Rat)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    This is Gs alpha with the C-terminal amino acids …

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

44. RET-SHC

    Plasmid

    #139617

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    RET (RET Human)
    

    Use

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. YFP Rab4DN

    Plasmid

    #99019

    Purpose

    Localization and visualization of the dominant negative form of Rab4

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    Rab4 DN (Rab4 Synthetic, Fly)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    S22N Dominant negative

    Promoter

    CMV

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26567

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons a10, 31a, 37b, and 46. Deletion…

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

47. TPI-PTC1

    Plasmid

    #130699

    Purpose

    Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC1

    Promoter

    Pcmv

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. HP21-TPI-WT-no_introns

    Plasmid

    #131044

    Purpose

    Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-Wildtype-no introns (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

    Plasmid

    #26569

    Depositor

    Diane Lipscombe

    Article

    Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

50. HA, EGFP Spastin M87 with exon 4, N386C

    Plasmid

    #167543

    Purpose

    For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

    Depositor

    Tarun Kapoor

    Article

    Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

    Insert

    Spastin M87 with exon 4 (SPAST Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    N386C

    Promoter

    CMV

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge