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Showing: 1001 - 1050 of 1662 results

  1. pWZ186

    Plasmid
    #163641
    Purpose
    rps-27>DHB::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB:2xmKate2::3xHA
    Use
    CRISPR
    Tags
    2xmKate2
    Expression
    Worm
    Mutation
    Promoter
    rps-27
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    163641-plasmid-map-sequence-id-324063

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  6. pCAG-HDAC4*

    Plasmid
    #71402
    Purpose
    CAG promoter, mammalian expression
    Depositor
    Bo Chen
    Article
    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.
    Insert
    HDAC4* (HDAC4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    After aa126, insertion leads to reading frame shi…
    Promoter
    CAG
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pCAGGS-FuG-B2

    Plasmid
    #67513
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.
    Insert
    Fusion Glycoprotein type B2
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67513-plasmid-map-sequence-id-381535

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  8. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120808-plasmid-map-sequence-id-235136

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  10. Synapsin sHRPb-NLG in FSW lentiviral vector

    Plasmid
    #73148
    Purpose
    Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPb-NLG (Nlgn1 Synthetic, Mouse)
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73148-plasmid-map-sequence-id-134528

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  14. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid
    #41733
    Purpose
    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Bacterial
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    T7
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41733-plasmid-map-sequence-id-56305

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  15. FLINC-AKAR1-CAAX

    Plasmid
    #87705
    Purpose
    Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1-CAAX
    Use
    Tags
    C-terminal targeting sequence from Kras
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87705-plasmid-map-sequence-id-176711

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  16. K9L mutant of H3K9me3 biosensor

    Plasmid
    #120807
    Purpose
    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    lysine 9 is mutated to leucine 9 on histone H3
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120807-plasmid-map-sequence-id-235134

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  17. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

    Plasmid
    #217893
    Purpose
    Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217893-plasmid-map-sequence-id-432152

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  20. VBS mutant of alpha catenin sensor

    Plasmid
    #71710
    Purpose
    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin ΔVBS FRET Sensor
    Use
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 316-405 in α -catenin…
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71710-plasmid-map-sequence-id-142366

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  22. Lyn-FLINC-EKAR1

    Plasmid
    #87707
    Purpose
    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87707-plasmid-map-sequence-id-171091

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  23. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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  26. pAW-BiP-Nb127D01-mCherry-KDEL

    Plasmid
    #171575
    Purpose
    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane
    Depositor
    Hidde Ploegh, Norbert Perrimon
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
    Use
    Tags
    BiP signal peptide and mCherry-KDEL
    Expression
    Insect
    Mutation
    Promoter
    fly actin5C promoter
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    171575-plasmid-map-sequence-id-339963

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  27. pAW-BiP-NbVHH05-mCherry-KDEL

    Plasmid
    #171574
    Purpose
    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane
    Depositor
    Hidde Ploegh, Norbert Perrimon
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
    Use
    Tags
    BiP signal peptide and mCherry-KDEL
    Expression
    Insect
    Mutation
    Promoter
    fly actin5C promoter
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171574-plasmid-map-sequence-id-336399

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  31. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217897-plasmid-map-sequence-id-432185

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  32. pWZ194

    Plasmid
    #163640
    Purpose
    rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Synthetic, Nematode)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Mutation
    Promoter
    rps-27
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    163640-plasmid-map-sequence-id-324064

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  33. S10A mutant of H3S10ph biosensor

    Plasmid
    #120810
    Purpose
    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Serine 10 is mutated to Alanine, and Threonine 3,…
    Promoter
    CMV
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120810-plasmid-map-sequence-id-235276

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  34. FLINC-AKAR1(TA)-CAAX

    Plasmid
    #87706
    Purpose
    Negative-control mutant of FLINC-AKAR1-CAAX.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1(TA)-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87706-plasmid-map-sequence-id-171088

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  35. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

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  36. pAWK61

    Plasmid
    #163642
    Purpose
    rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Human, Nematode)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Mutation
    Promoter
    rps-27
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    163642-plasmid-map-sequence-id-362086

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  37. pMT-HGP-v3-NbVHH05-hIgG

    Plasmid
    #171565
    Purpose
    Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells
    Depositor
    Hidde Ploegh, Norbert Perrimon
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
    Use
    Tags
    BiP signal peptide and hIgG1-hinge-N297A-TEV site…
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171565-plasmid-map-sequence-id-336181

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  38. pVD1 Multiplexing Edit En-1 (GB2242)

    Plasmid
    #160564
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    Multiplexing Edit (En-1)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160564-plasmid-map-sequence-id-319675

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  40. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

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  46. pVD1 tRNA-gRNA E4-En-1 (GB2243)

    Plasmid
    #160565
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E4-En-1 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160565-plasmid-map-sequence-id-319679

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  47. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid
    #91819
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91819-plasmid-map-sequence-id-176494

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  48. JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

    Plasmid
    #156417
    Purpose
    8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    murine Dll4 F2-6/F8 ETS site B/ site #2, 8X
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    E1b min pro/b-globin intron
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156417-plasmid-map-sequence-id-308709

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  50. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Use
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    66080-plasmid-map-sequence-id-436984

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Showing: 1001 - 1050 of 1662 results