Skip to main content
Addgene
Showing: 1001 - 1050 of 17130 results

  3. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    82451-plasmid-map-sequence-id-156993

    Enlarge

  8. piRES-puro-HE4

    Plasmid
    #25727
    Depositor
    Ronny Drapkin
    Insert
    WAP four-disulfide core domain 2 (WFDC2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Available since
    Oct. 22, 2010
    Availability
    Academic Institutions and Nonprofits only

  28. pLD-puro-Cc-IFIT5_K197R-VA

    Plasmid
    #191223
    Purpose
    Lentiviral expression of human IFIT5_K197R in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    changed Lysine 197 to Arginine (K197R)
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191223-plasmid-map-sequence-id-467902

    Enlarge

  29. pLD-puro-Cc-IFIT5_K206R-VA

    Plasmid
    #191224
    Purpose
    Lentiviral expression of human IFIT5_K206R in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    3xFlag-TEVx2-6xHis-Strep x2 tag and VA tag
    Expression
    Mammalian
    Mutation
    changed Lysine 206 to Arginine (K206R)
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191224-plasmid-map-sequence-id-438791

    Enlarge

  30. pLD-puro-Cc-IFIT5_WT-VA

    Plasmid
    #191221
    Purpose
    Lentiviral expression of human IFIT5_WT in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191221-plasmid-map-sequence-id-433869

    Enlarge

  31. pLD-puro-Cc-IFIT5_K160R-VA

    Plasmid
    #191222
    Purpose
    Lentiviral expression of human IFIT5_K160R in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    changed Lysine 160 to Arginine (K160R)
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191222-plasmid-map-sequence-id-467897

    Enlarge

  32. pLenti Puro HA-Ubiquitin-KO

    Plasmid
    #236292
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin KO mutant
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin KO (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence containing KO mu…
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236292-plasmid-map-sequence-id-468935

    Enlarge

  33. pLenti Puro HA-Ubiquitin-WT

    Plasmid
    #236289
    Purpose
    Lentiviral vector expressing HA-tagged wild type ubiquitin
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236289-plasmid-map-sequence-id-467836

    Enlarge

  38. Lenti-SAM-Puro

    Plasmid
    #198713
    Purpose
    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198713-plasmid-map-sequence-id-402626

    Enlarge

  39. pCMV-PuroR-2A-MLH1-SB

    Plasmid
    #228871
    Purpose
    Plasmid for MLH1-SB overexpression
    Depositor
    Sangsu Bae
    Insert
    PuroR-2A-MLH1-SB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228871-plasmid-map-sequence-id-457184

    Enlarge

  41. qTAG-KO-Puro-TP53

    Plasmid
    #227319
    Purpose
    Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227319-plasmid-map-sequence-id-451734

    Enlarge

  42. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212627-plasmid-map-sequence-id-420278

    Enlarge

  43. CROPseq-Puro-12xMS2

    Plasmid
    #212628
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212628-plasmid-map-sequence-id-422312

    Enlarge

  44. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212626-plasmid-map-sequence-id-420274

    Enlarge

  48. pCSIIpuro-BTC-ScNeo

    Plasmid
    #209898
    Purpose
    To monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    BTC-ScNeo (BTC Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209898-plasmid-map-sequence-id-416606

    Enlarge

  49. pCSIIpuro-EPGN-ScNeo

    Plasmid
    #209899
    Purpose
    To monitor the status of Epigen, the plasmid encodes a recombinant Epigen fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EPGN-ScNeo (EPGN Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209899-plasmid-map-sequence-id-416612

    Enlarge

  50. pCSIIpuro-NRG1-ScNeo

    Plasmid
    #209900
    Purpose
    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    NRG1-ScNeo (NRG1 Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209900-plasmid-map-sequence-id-416651

    Enlarge

Showing: 1001 - 1050 of 17130 results