167,150 results
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Plasmid#84028PurposeExpression of GFP tagged mutant BuGZ in human cellsDepositorInsertBuGZ (ZNF207 Human)
UseLentiviralTagsTurboGFPExpressionMammalianMutationdelta 357−373; Q394RPromoterUCOE / SFFVAvailable SinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)
Plasmid#225752PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer
Plasmid#176588PurposeEndogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Delaware Bay) locus
ExpressionBacterialMutationReplaced spacer in CRISPR array with Fn spacerAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-2-human RNF146 full length
Plasmid#132610Purposeexpresses human RNF146 full length in E. coliDepositorInsertRNF146 (RNF146 Human)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX028
Plasmid#167151PurposeMoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plantsDepositorInsertp35s-nnHispS-ocsT
ExpressionPlantPromoterp35sAvailable SinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-eGFP-hcGAS
Plasmid#108674PurposeFor production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGASDepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPlum-pBAD
Plasmid#54564PurposeLocalization: Protein Expression Vector , Excitation: 590, Emission: 649DepositorTypeEmpty backboneTags6xHis and mPlumExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd
Plasmid#214370PurposeCloning vector with BlasticidinDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RFP-p2a-postASAP
Plasmid#178793PurposeExpresses voltage sensor targeted to dendrites and spines along with RFPDepositorInsertFingR.PSD95, ASAP2f and TagRFP
TagsTagRFPExpressionMammalianMutationmutations in amino acids L146G, S147T N149R, S150…PromoterCAGAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFBOS_CreIRESpuro
Plasmid#183812PurposeExpresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoterDepositorInsertCre recombinase
UseSynthetic BiologyTagsNLSExpressionMammalianAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mito-RA
Plasmid#209866PurposeDimerization dependent fluorescent protein R anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddRFP A
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC hTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214996PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C5_15
Plasmid#60322PurposeThis plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.DepositorInsertNon active element (nearest TSS PHLDA1) (PHLDA1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC30A1
Plasmid#132137PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC30A1 (SLC30A1 Human)
ExpressionMammalianAvailable SinceNov. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pABE8e-NG-403Q-Nterm-AAV
Plasmid#194651PurposeN-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in miceDepositorInsertABE8e-NG N-term
UseAAVTagsBPNLS and NpuN spilt inteinPromoterTNNT2Available SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-NG-403Q-Cterm-AAV
Plasmid#194652PurposeC-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in miceDepositorInsertABE8e-NG C-term
UseAAVTagsBPNLS and NpuC split inteinPromoterTNNT2Available SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PH-PLCdelta1
Plasmid#66841Purposeexpression of near-infrared PI(4,5)P2 biosensorDepositorInsertPH-PLCdelta1
TagsiRFPExpressionMammalianPromoterCMVAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon
ExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DL017:CMV::HaloTag-iPAK4
Plasmid#177882PurposeHT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backboneDepositorInsertiPAK4
UseLentiviralTagsHaloTagExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX029
Plasmid#167152PurposeMoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plantsDepositorInsertp35s-nnH3H-ocsT
ExpressionPlantPromoterp35sAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8327 pLIX YAP1 S127A
Plasmid#184528PurposeExpression of YAP1 S127ADepositorAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
ExpressionInsectPromoterp10Available SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag
Plasmid#209651PurposeExpression of iATPSnFR2, high affinityDepositorInsertiATPSnFR2.S29W.HaloTag
UseAAVMutationS29W.A95KPromoterCAGAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-actin E6 E7
Plasmid#13712DepositorAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
hBax C3-EGFP
Plasmid#19741DepositorAvailable SinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense
Plasmid#46839PurposeExpress cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translatedDepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB4581
Plasmid#171124PurposeA kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.DepositorInsert3_sAID
UseOtherMutationnon applicableAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Basigin_iso2
Plasmid#221416PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertBasigin_iso2 (BSG Human)
ExpressionMammalianAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Kl-EGFP
Plasmid#45532DepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFREE019Env
Plasmid#137670PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
ExpressionMammalianAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVT36b
Plasmid#184437PurposeCRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmTurqoise KIF1A shRNA
Plasmid#102849PurposeGenerates shRNA specific to Rat KIF1A along with cell fill pmTurqoiseDepositorAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CDKN2A-L78Hfs*41
Plasmid#116222PurposeLentiviral expression of CDKN2A L78Hfs*41DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-SpeI
Plasmid#87331PurposeExpression of shRNA targeting at Nup93DepositorInsertshRNA against human Nup93
UseRNAiExpressionMammalianPromoterH1Available SinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-DAPK1
Plasmid#23480DepositorInsertDAPK1 (DAPK1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDACR3882 [rab-3p::HYlight::let-858utr]
Plasmid#207666PurposeExpresses worm-optimized HYlight in C. elegans neuronsDepositorInsertHYlight Biosensor
ExpressionWormPromoterrab-3Available SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDACR3883 [rab-3p::HYlight-RA::let-858utr]
Plasmid#207667PurposeExpresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neuronsDepositorInsertHYlight-RA [T152E]
ExpressionWormPromoterrab-3Available SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only