165,187 results

1. pLenti-eIF6-3XFlag

   Plasmid

   #242188

   Purpose

   Express eIF6-3xFlag in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

   Insert

   eIF6 (EIF6 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Promoter

   CMV

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP12320 - pAAV-AiE0475m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2320)

   Plasmid

   #229885

   Purpose

   AiE0475m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-FLEX-Positron2-ST-WPRE

   Plasmid

   #239080

   Purpose

   AAV-mediated expression of positive-going voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Eric R. et al Janelia Figshare 2023

   Insert

   Positron2-ST
   

   Use

   AAV and Cre/Lox

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   R78K N81D D92N W178F

   Promoter

   hSynapsin1

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-CB-Cdkn1a

   Plasmid

   #228914

   Purpose

   Expression CDKN1A in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   Cdkn1a (Cdkn1a Mouse)
   

   Expression

   Mammalian

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

   Plasmid

   #233353

   Purpose

   CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   NES-Kinprola_PKA-mEGFP
   

   Tags

   NES and mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-KDEL_SBP-mCherry-CCR1

   Plasmid

   #222311

   Purpose

   Synchronize the trafficking of CCR1 from the ER.

   Depositor

   Franck Perez

   Article

   Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

   Insert

   Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGIG-CDKN1A

   Plasmid

   #228912

   Purpose

   Expression CDKN1A in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   Cdkn1a (Cdkn1a Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH4783

   Plasmid

   #200785

   Purpose

   Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   snb-1 (snb-1 Nematode)
   

   Tags

   pHiuorin

   Expression

   Worm

   Promoter

   Pnpr-4

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pQE60 R196A wgMDH

   Plasmid

   #204271

   Purpose

   Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R196A

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR032

    Plasmid

    #198773

    Purpose

    Stabilized step function Opsins ChR2 variant

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJR034

    Plasmid

    #198829

    Purpose

    Step-Waveform Inhibitory Channelrhodopsin

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::SwiCHR-TS-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJR033

    Plasmid

    #198822

    Purpose

    Chloride-conducting channelrhodopsin

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::UAS-iC1-c2-TS-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-TNFSF13B-COMP5AP-AviTag-9xHis

    Plasmid

    #157213

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TNFSF13B (TNFSF13B Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RCAS.Sfi

    Plasmid

    #196377

    Purpose

    Empty avian expression vector RCAS with modifications for fixed orientation cloning into SfiI sites

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Type

    Empty backbone

    Use

    Retroviral; Avian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJH3717

    Plasmid

    #191477

    Purpose

    Pacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Arch (arch-1 Nematode)
    

    Tags

    wCherry

    Expression

    Worm

    Promoter

    Pacr-2s

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPHK1 gRNA (BRDN0001148403)

    Plasmid

    #76815

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ203 (pMDC32-Ubi1)

    Plasmid

    #86207

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Expression

    Plant

    Promoter

    Maize ubiquitin 1 (ZmUbi1)

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-C1-p63RhoGEF

    Plasmid

    #67896

    Purpose

    Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-p63RhoGEF
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMKO.1 puro

    Plasmid

    #8452

    Purpose

    Retroviral vector for shRNA expression.

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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21. iRFP670-PIP4K2C

    Plasmid

    #202750

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP670:PIP4K2C (PIP4K2C Human)
    

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. HBx

    Plasmid

    #65463

    Purpose

    expresses HBx in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al Hepatology. 2004 Jun;39(6):1683-93.

    Insert

    HBV X protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. Raf-1 GST RBD 1-149

    Plasmid

    #13338

    Depositor

    Channing Der

    Article

    Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

    Insert

    Raf-1 (RAF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. ABE-NW-NL

    Plasmid

    #240621

    Purpose

    Adenine base editor with narrowed editing window

    Depositor

    Tingting Jiang

    Article

    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

    Insert

    TadA8e(N108Q,M151C,Q154Y)-nCas9 (D10A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    TadA8e(N108Q,M151C,Q154Y), Cas9(D10A)

    Available Since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid

    #68214

    Purpose

    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    P19 silencing suppressor
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.4-GFP-TEV-B55

    Plasmid

    #217378

    Purpose

    expresses GFP tagged B55 in mammalian cells; tev cleavable

    Depositor

    Wolfgang Peti

    Article

    Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

    Insert

    B55 (PPP2R2A Human)
    

    Tags

    EGFP TEV

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBECAb-apr

    Plasmid

    #122001

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLenti-TripleColour-StAR

    Plasmid

    #222695

    Purpose

    Competition assay

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP, miRFP703, dTomato

    Expression

    Mammalian

    Mutation

    PGK, TagBFP, dTomato without BsmBI cutsite

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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