163,777 results

1. pAAV-C2-mKate

   Plasmid

   #208792

   Purpose

   For AAV production to express C2-mKate in astrocytes

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2-mKate (Mfge8 Mouse)
   

   Use

   AAV

   Tags

   mKate

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Myc-FOXA1

   Plasmid

   #219394

   Purpose

   Express FOXA1 in mammalian cells

   Depositor

   Yoichi Furukawa

   Article

   Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

   Insert

   FOXA1 (FOXA1 Human)
   

   Use

   Tags

   N terminal Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-N1_SEPT2

   Plasmid

   #71547

   Purpose

   mammalian expression of Sept 2 with a GFP fusion

   Depositor

   Elias Spiliotis

   Article

   Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97.

   Insert

   Septin 2 (Septin2 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM2195

   Plasmid

   #40245

   Depositor

   Susan Golden

   Article

   Mackey et al Methods Mol Biol. 2007;362:115-29.

   Insert

   PpsbAI::luxCDE, PpsbAI::luxAB
   

   Use

   Cyanobacteria cloning vector

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX NPC1-FLAG-TiD

   Plasmid

   #199748

   Purpose

   Lentiviral construct to stably express NPC1 TurboID

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Tags

   Flag and TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-FOXL1

   Plasmid

   #153134

   Purpose

   Expression of Flag tagged human FOXL1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXL1 (FOXL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLG001 (empty vector)

   Plasmid

   #157879

   Purpose

   Empty vector backbone for defense systems

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   None
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p368_DG

   Plasmid

   #234691

   Purpose

   Golden gate domesticated pET28b with 3C-His6

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   3C-His6

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p344_DG

   Plasmid

   #234674

   Purpose

   Golden gate domesticated pET28b with His6-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His6-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid

    #140974

    Purpose

    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai2-Rluc8 (GNAI2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZmUBQ:GW

    Plasmid

    #132358

    Purpose

    Expression of CDS from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-Tau-mCherry-RING

    Plasmid

    #233689

    Purpose

    Inducible expression of Tau-mCherry-RING

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Tau-mCherry-RING
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUCas9Cherry

    Plasmid

    #70182

    Purpose

    Expresses Cas9 with fluorescent Cherry reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    humanised S.pyogenes cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dCas13-M3nls

    Plasmid

    #155366

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TLR5-CFP

    Plasmid

    #13019

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 5 (TLR5 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti Dest BlastR R1-R2

    Plasmid

    #84574

    Purpose

    Gateway destination vector expressing Blasticidin resistance under a PGK promoter

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Gateway

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-3XMyc-mTrim25

    Plasmid

    #51394

    Depositor

    Narry Kim

    Article

    Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

    Insert

    Trim25 (Trim25 Mouse)
    

    Use

    Lentiviral

    Tags

    3XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. M13-kmR

    Plasmid

    #206861

    Purpose

    M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

    Depositor

    Irene Chen

    Article

    Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

    Insert

    Kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RBM14_pLX307

    Plasmid

    #98367

    Purpose

    Lentiviral expression of RBM14

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    RBM14 (RBM14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. E.coli RaPID plasmid

    Plasmid

    #107251

    Purpose

    Expresses the E.coli RaPID labeling protein

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    E.coli BirA*
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pICSL4723-P1

    Plasmid

    #86173

    Purpose

    LevelP cloning Vector

    Depositor

    Mark Youles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMito-mCherry-FRB

    Plasmid

    #59352

    Purpose

    Expresses mCherry-tagged MitoTrap in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Type

    Empty backbone

    Use

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. XEC

    Plasmid

    #233341

    Purpose

    expressing SARS-CoV-2 XEC spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XEC) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T22N, F59S on the basis of KP.3

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TALED_Left-ND1-AD-E1347A

    Plasmid

    #183894

    Purpose

    Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBad-EBFP2

    Plasmid

    #14891

    Purpose

    Bacterial expression of EBFP2 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Has Service

    Cloning Grade DNA

    Insert

    EBFP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Promoter

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hCCR5-mTFP1

    Plasmid

    #110193

    Purpose

    Encodes for human CCR5 coding sequence tagged with the mTFP1 FP

    Depositor

    Sergi Padilla Parra

    Article

    Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

    Insert

    hCCR5 (CCR5 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-Lyn-ICUE3

    Plasmid

    #61623

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    Lyn-ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid

    #52093

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCINeo-myc-Itch (C822S)/2860

    Plasmid

    #11428

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Itch (Itch Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    cysteine at 822 mutated to serine

    Promoter

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NeonGreen-C1-ORP5

    Plasmid

    #220071

    Purpose

    overexpresses ORP5 with a neonGreen fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL5 (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GLUT1-GFP

    Plasmid

    #89440

    Purpose

    GLUT1 construct

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    GLUT1 (Slc2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237)

    Plasmid

    #191707

    Purpose

    AI plasmid ALIAS: AiP12237. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    none

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

    Plasmid

    #159915

    Purpose

    Mutagenesis of Rosa26

    Depositor

    Larry Zweifel

    Insert

    Rosa26 (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AChE-pcDNA

    Plasmid

    #106442

    Purpose

    Mammalian expression of murine acetylcholinesterase

    Depositor

    Georg Weber

    Article

    Syed et al Clin Exp Metastasis. 2008;25(7):787-98.

    Insert

    acetylcholinesterase (ACHE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lenti-sh1368 knockdown c-myc

    Plasmid

    #29435

    Depositor

    Bob Eisenman

    Article

    Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

    Insert

    c-Myc shRNA (MYC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP and WPRE

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid

    #51006

    Purpose

    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-cerFP
    

    Use

    Lentiviral

    Tags

    histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EB3-GFP

    Plasmid

    #190164

    Purpose

    Expresses EB3-GFP in mammalian cells

    Depositor

    Anna Akhmanova

    Article

    Stepanova et al J Neurosci. 2003 Apr 1;23(7):2655-64.

    Insert

    EB3-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pC1-CMV-mito-DAAO

    Plasmid

    #141132

    Purpose

    Expresses DAAO with mitochondrial localization signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Insert

    mito-DAAO
    

    Use

    Tags

    2 mitochondrial localization signals and 7xHis

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge