We narrowed to 171,035 results for: Gene
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Plasmid#13984DepositorInsertgoosecoid (GSC Chicken)
UseRetroviral; Avian expressionAvailable SinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMM0647
Plasmid#129008PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Fos DD T325A T331A
Plasmid#8973DepositorAvailable SinceSept. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159673PurposePlasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertRcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyMutationAll BsaI, Esp3I and BpiI restriction sites were r…Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlluciferase
Plasmid#63853PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVMG0173_Cq-Actin5c-Cas9
Plasmid#169345PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0173_Cq-Actin5c-Cas9
UseCRISPRExpressionInsectPromoterCPIJ009808Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
CLDN8_pCSdest
Plasmid#53811PurposeDepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTNos:BASTA:PNos (GB0023)
Plasmid#68211PurposeProvides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.DepositorInsertbar gene
UseUnspecifiedMutationBsaI and BsmBI sites removedPromoterPNosAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-Rev
Plasmid#112811PurposegRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S127/387A
Plasmid#33079DepositorAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVMG0212_Cq-nanos-Cas9
Plasmid#169348PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0212_Cq-nanos-Cas9
UseCRISPRExpressionInsectPromoterCPIJ011551Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
Plasmid#169370PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
UseCRISPRExpressionInsectPromoterCPIJ039596Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0213_Cq-vasa-Cas9
Plasmid#169347PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0213_Cq-vasa-Cas9
UseCRISPRExpressionInsectPromoterCPIJ009286Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH371-SUP45-N58A
Plasmid#29378DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationN58A mutant gene including genomic sequence 1432 …Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLN481 (FuGW-G8p-IL12-1Pe)
Plasmid#105191PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-IL12-1Pe
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/UAA-mEGFP-IRES-mCherry
Plasmid#49234PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-RAP1
Plasmid#84525PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
D92_Ld_360170
Plasmid#135905PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserthypothetical protein, conserved
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N-Myc-Flag-BirA-hPOT1
Plasmid#166409Purposeexpress BirA-hPOT1 in mammalian cellsDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN3-Ndelta
Plasmid#96981PurposeExpress N-terminal truncated Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
Tags6xHis and GFPExpressionPlantMutationA segment of 507 nucleotides are deleted from the…Promoter35SAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) HIS-tag pET
Plasmid#8543DepositorInsertHOXB13 (HOXB13 Human)
TagsHis and T7ExpressionBacterialMutationmakes protein by Western and by transcription/tra…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49228PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNArodA
Plasmid#149656Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATF4 8: (SL-)5’ATF4.GFP
Plasmid#21855DepositorAvailable SinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
LacD.1_WT_pET21a
Plasmid#29789DepositorInsertTagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))
TagsHisExpressionBacterialAvailable SinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
HOXC6-pCT
Plasmid#21000DepositorAvailable SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast
Plasmid#166412Purposeexpress hPOT1 K90E in mammalian cellsDepositorInserthPOT1 K90E (POT1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationK90EPromoterEF-1aAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_IRES_hygro
Plasmid#166406Purposeexpress hPOT1 in mammalian cellsDepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
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3087 pBS Bcl-2-Ig
Plasmid#8809DepositorInsertBcl-2-Ig (BCL2 Human)
ExpressionBacterialAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
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pSKB3-SaGGR-I206F-L377H (JBEI-7202)
Plasmid#110152PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant geneDepositorInsertGGR
TagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-I206F-L377H mutant genePromoterT7Available SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pTH375-SUP45-P174Q
Plasmid#29383DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationP174Q mutant gene including genomic sequence 1432…Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG
Plasmid#129758PurposeDox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMammalianMutationchanged Glutamic acid 36 to Alanine and Leucine 3…PromoterTRE3Gs and Tet inducible promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Py2087ACR1
Plasmid#153032PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertPy2087ACR1
TagsmCherryExpressionMammalianPromoterCMV (+ enhancer)Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAPi
Plasmid#127548PurposeEmpty control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertAdenine Phosphorybosyltransferase transcript segment
UseRNAi; Empty controlPromoterUbiquitinAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRYAA_pCSdest
Plasmid#53927DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
drd4a_L (TAL 3006)
Plasmid#35998DepositorInsertTAL array targeting drd4a (drd4a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) Myc-tag-GST
Plasmid#8683DepositorInsertHOXB13 (HOXB13 Human)
TagsGST and MycExpressionBacterialMutationmakes protein by westernAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFusX4-35
Plasmid#63444PurposeEncodes RVD positions 10-12 in TALEN assemblyDepositorInsertNN NI NN
UseTALENMutationnoneAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7g1(K165A)
Plasmid#14675DepositorAvailable SinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-4EHP(K121/130/134/222R)
Plasmid#17353DepositorAvailable SinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-145-LUC
Plasmid#46866Purposemurine 24p3 minimal promoter as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferasePromoter24p3 promoter (inactive)Available SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS chick ER-81 [TJ#50]
Plasmid#16274DepositorInsertER81 (ETV1 Chicken)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
ATF4 19: (SL)RLuc.5’ATF4.GFP
Plasmid#21865DepositorAvailable SinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only