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We narrowed to 167,150 results for: Gene

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Showing: 10651 - 10700 of 167150 results

  2. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid
    #140200
    Purpose
    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140200-plasmid-map-sequence-id-310179

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  8. pIhh1.3K-Luc

    Plasmid
    #53604
    Purpose
    Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Available Since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49218
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    Viral LTR (or CMV)
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49218-plasmid-map-sequence-id-73625

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  15. pGEM-myr-tdTomato-SV40pA-FKF

    Plasmid
    #119867
    Purpose
    Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    2x myristoylation
    Expression
    Bacterial
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119867-plasmid-map-sequence-id-239422

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  20. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129008-plasmid-map-sequence-id-259450

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  27. EC71139

    Plasmid
    #154062
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    pZmUbi
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BpiI restriction sites were r…
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154062-plasmid-map-sequence-id-312952

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  32. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  33. pTNos:BASTA:PNos (GB0023)

    Plasmid
    #68211
    Purpose
    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    bar gene
    Use
    Unspecified
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    PNos
    Available Since
    Oct. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68211-plasmid-map-sequence-id-119063

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  34. AEB-No-aptamer-controlluciferase

    Plasmid
    #63853
    Purpose
    This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.
    Depositor
    Howard Salis
    Article
    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv
    Insert
    No-aptamer control mRNA
    Use
    Synthetic Biology
    Promoter
    tac
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    63853-plasmid-map-sequence-id-106576

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  44. pWPT-/UAA-mEGFP-IRES-mCherry

    Plasmid
    #49234
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49234-plasmid-map-sequence-id-73657

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Showing: 10651 - 10700 of 167150 results