We narrowed to 16,985 results for: She

1. pGEX6P-1 BICC FL

   Plasmid

   #112998

   Purpose

   For protein expression and purification of full-length Drosophila BicC

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length BicC (BicC Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CDk4-luciferase fragment B

   Plasmid

   #86659

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   generated by cleavage at the KpnI site from 880 (…

   Promoter

   CDK7

   Available since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LP451

   Plasmid

   #90183

   Purpose

   pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 841-1426

   Depositor

   Lotte Pedersen

   Article

   Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

   Insert

   NPHP4 (NPHP4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   aa residues 841-1426

   Promoter

   CMV

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET151-Vps4(101-437) L151D

   Plasmid

   #87736

   Purpose

   Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Leu 151 to Asp

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. csd2_121-308/pET21a(+)

   Plasmid

   #83293

   Purpose

   csd2, construct: 121-308, pET21a(+) no-tag

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1544
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-120

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. csd2_140-251/pET21a(+)

   Plasmid

   #83294

   Purpose

   csd2, construct:140-251, pET21a(+) C-tag

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1544
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-139 and 252-308

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. csd1_54-312/pET21a(+)

   Plasmid

   #83291

   Purpose

   csd1, construct: 54-312, pET21a(+) C-tag

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1543
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-53

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. csd2_121-308/pET28b(+)

   Plasmid

   #83292

   Purpose

   csd2, construct: 121-308, pET28b(+) NC-tag

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1544
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-120

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. csd1_125-312/pET28b(+)

   Plasmid

   #83290

   Purpose

   csd1, construct: 125-312, pET28b(+) NC-tag

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1543
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-124

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB-CAG-Mbd3b-pA-pgk-bsd

    Plasmid

    #74920

    Purpose

    Mammalian expression of Mbd3b

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Insert

    Mnd3b (Mbd3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pPB-CAG-Mbd3c-pA-pgk-bsd

    Plasmid

    #74921

    Purpose

    Mammalian expression of Mbd3c

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Insert

    Mbd3c (Mbd3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

12. mCardinal-Cx26-7

    Plasmid

    #56156

    Purpose

    Localization: Gap Junctions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Cx26
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. mCardinal-CAF1-C-10

    Plasmid

    #56153

    Purpose

    Localization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    CAF1
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pLA CMV N-Flag RALB V23 R47

    Plasmid

    #50983

    Purpose

    Expresses RALB-V23/R47 in mammalian cells

    Depositor

    Anna Sablina

    Article

    Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

    Insert

    RALB (RALB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    G23V, K47R

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLA CMV N-Flag RALB V23 R160

    Plasmid

    #50982

    Purpose

    Expresses RALB-V23/R160 in mammalian cells

    Depositor

    Anna Sablina

    Article

    Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

    Insert

    RALB (RALB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    G23V K160R

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pVRa02_915

    Plasmid

    #49653

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF02_915
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe_puro_DEST_MYNN

    Plasmid

    #45260

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MYNN (MYNN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81

    Plasmid

    #45293

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    deleted 81 N-terminal amino acids

    Promoter

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pWzl_neo_DEST_flag_SKIL

    Plasmid

    #45303

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaC27

    Plasmid

    #45292

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    deleted 27 c-terminal amino acids

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN149

    Plasmid

    #45294

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

22. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

    Plasmid

    #224536

    Purpose

    A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-FLAG- NLS-mScarlet-I3
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. IKK-2 S177E S181E

    Plasmid

    #11105

    Purpose

    Mammalian expression of constitutively active human IKK-2

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S177E and S181E, constitutively active

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDF-Frb-v1

    Plasmid

    #201923

    Purpose

    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (modified), T7 (modified, moderate), T7 (modif…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSCV-miR30-Vector

    Plasmid

    #171194

    Purpose

    Retroviral vector for U6 promoter driven expression empty miR30 based shRNA (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    shRNA empty vector
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pME-EGFP-P2A-iCre (JDW 921)

    Plasmid

    #224519

    Purpose

    A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EGFP-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-mTurquoise-P2A-iCre (JDW 920)

    Plasmid

    #224518

    Purpose

    A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mTurquoise-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHiUGE smFP-V5 donor (all-in-one) ORF2

    Plasmid

    #200388

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. FH-TET1-pEF

    Plasmid

    #49792

    Purpose

    This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

    Depositor

    Anjana Rao

    Article

    Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

    Insert

    Tet methylcytosine dioxygenase 1 (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB-TO-oNGN2

    Plasmid

    #198397

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

    Promoter

    CAG, EF1a, and TRE3G

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

    Plasmid

    #222852

    Purpose

    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    IgG1_Fc

    Expression

    Mammalian

    Mutation

    L234A, L235A, P329G

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral vector

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pME-FastFUCCI (JDW 1488)

    Plasmid

    #242556

    Purpose

    Gateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle state

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
    

    Use

    Gateway subcloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

    Plasmid

    #229807

    Purpose

    A CAGGS driven tet-on transactivator with an nls-EGFP reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    rtTA-IRES-nls-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CYFIP1.mCherry

    Plasmid

    #122051

    Purpose

    Expresses human CYFIP1 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLX304_zeo_eGFP

    Plasmid

    #160095

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

    Plasmid

    #226577

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    I178A, L182A, L186A, V190A

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Expression

    Mutation

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pUC19/PLIN2-GFP

    Plasmid

    #202194

    Purpose

    knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3-FLAG-MTOR-L1460P

    Plasmid

    #69006

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-L1460P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Leucine 1460 to Proline

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218863

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. FLAG-PRMT6

    Plasmid

    #164697

    Purpose

    Expression of PRMT6 with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

    Insert

    PRMT6 (PRMT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Flag-Axin1

    Plasmid

    #109370

    Purpose

    Transient over-expression of human Axin1

    Depositor

    Mariann Bienz

    Article

    Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LentiGuide Puro-P2A-EGFP

    Plasmid

    #137729

    Purpose

    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. TK-GFP-Nluc-P2A-neo-TK

    Plasmid

    #134896

    Purpose

    Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Insert

    TK-GFP-Nluc-P2A-Neo-TK
    

    Use

    Cryptosporidium expression

    Tags

    Expression

    Mutation

    Promoter

    Cryptosporidium actin and enolase

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

    Plasmid

    #192148

    Purpose

    T cell CRISPR AAV vector

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge