173,273 results

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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KMT2A_MLLT1

Plasmid

#205842

Purpose

Express mEGFP-tagged fusion protein, KMT2A_MLLT1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KMT2A_MLLT1 (KMT2A, MLLT1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJL-TRBO-G

Plasmid

#80083

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Insert

GFP

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.2

Plasmid

#172320

Purpose

expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.2 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

Promoter

CMV-F

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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ptRNA100

Plasmid

#154133

Purpose

Expresses a set of tRNAs in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

tRNAs operon under control of Ptac promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_3xFLAG_NLS_ISCro4_NLS_T2A_puroR_SV40_polyA

Plasmid

#251494

Purpose

Expression of a human codon optimised ISCro4 bridge recombinase

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge recombinase

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLPT119

Plasmid

#85526

Purpose

Repressilator without degradation tags, with integrated reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

Plasmid

#173899

Purpose

The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

Depositor

Jason W Chin

Article

Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

Inserts

ThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1389 IBB-GFP-mCherry3E

Plasmid

#118803

Purpose

Fluorescence-based TDP43-dependent splicing reporter

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

Synthetic

Expression

Mammalian

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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syntaxin3-myc-myc-His

Plasmid

#12372

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Article

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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nuclear cpVenus control

Plasmid

#186790

Purpose

Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

nuclear cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8f-WPRE

Plasmid

#162376

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pmStayGold_mStayGold_I3

Plasmid

#239339

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mStayGold

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-MCS-WPRE-UbC-mCherry

Plasmid

#211799

Purpose

Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

multiple cloning site
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AA068

Plasmid

#215994

Purpose

Fragmid fragment: (Selection) puromycin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; PuroR_v1.2

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSGKP-spe

Plasmid

#117234

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP1-SEP-mRuby3

Plasmid

#200939

Purpose

endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

LAMP1 (LAMP1 Human)
SEP-mRuby3

Use

Lentiviral

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pER-mScarlet3_N1

Plasmid

#189772

Purpose

Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

ER-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Hnf4a

Plasmid

#33002

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pBMN-I-GFP

Plasmid

#1736

Purpose

This retroviral vector has an IRES-GFP downstream of the cloning sites

Depositor

Garry Nolan

Insert

IRES-GFP

Use

Retroviral

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-FBL

Plasmid

#63672

Purpose

PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

EGFP-2A-PuroR

Use

CRISPR

Promoter

Promoterless

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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ampkar

Plasmid

#35097

Purpose

A fluorescent reporter of AMPK activity and cellular energy stress

Depositor

Lewis Cantley

Article

Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

Insert

ampkar

Tags

ECFP and Venus

Expression

Bacterial

Mutation

na

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pGRB

Plasmid

#71539

Purpose

for expression of gRNA in E. coli

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Type

Empty backbone

Expression

Bacterial

Promoter

J231196

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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