We narrowed to 18,870 results for: IRE

1. eGFP-RAPH1-F1

   Plasmid

   #194860

   Purpose

   Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids 1-535

   Promoter

   CMV

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUE1-TSP-ORF68 R443A

   Plasmid

   #162652

   Purpose

   Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acid R443 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaG-17

   Plasmid

   #172747

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-17
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-MYB-3'UTR

   Plasmid

   #25798

   Depositor

   Judy Lieberman

   Article

   Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

   Insert

   3'UTR of human MYB (MYB Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL2-200bp

   Plasmid

   #16293

   Depositor

   Wafik El-Deiry

   Article

   Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

   Insert

   p53 promoter fragment (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   fragment -188 to +12 relative to first transcript…

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pHisAlix

   Plasmid

   #42576

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Bacterial

   Mutation

   silent mutation A1617G (numbering in ALIX gene)

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pFRTpsiCHECK

   Plasmid

   #154454

   Purpose

   Expression of renilla luciferase and firefly luciferase

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   Renilla luciferase, firefly luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBluescript-mouse eIF2C3

   Plasmid

   #50355

   Purpose

   Mouse eIF2C3 cDNA clone

   Depositor

   Kumiko Ui-Tei

   Article

   Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

   Insert

   eIF2C3 (Ago3 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGK401red-hENT1

   Plasmid

   #166028

   Purpose

   Expresses human equilibrative nucleoside transporter 1 (hENT1)

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   human equilibrative nucleoside transporter 1 (SLC29A1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPGK1

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsXRN1-del1174-1649_AH

    Plasmid

    #148904

    Purpose

    Mammalian Expression of HsXRN1-del1174-1649

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsXRN1-del1174-1649 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-TO-HA-SH3BP1 (RNAi-res)

    Plasmid

    #136645

    Purpose

    Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1 (RNAi resistant) (SH3BP1 Human)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p1977 (pUC19-PTDH3-PTEF1)

    Plasmid

    #78230

    Purpose

    Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Bidirectional promoters pTDH3-pTEF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pDBEcR/Bla

    Plasmid

    #201556

    Purpose

    Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-Flag-hsDicer (E1564A)

    Plasmid

    #41587

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E1564A

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCRII-Topo Plexin D1 in situ probe

    Plasmid

    #45621

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Plexin D1 in situ probe (Plxnd1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 5751-6406 of NM_026376.3

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+)-TTF-1-HDD

    Plasmid

    #52063

    Purpose

    It encodes a mutant of TTF-1 in which the homeodomain is deleted

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    Thyroid Transcription Factor 1 (NKX2-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Homeodomain deleted

    Promoter

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT/TO-p97wt-mycStrep

    Plasmid

    #31837

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pAc5.1B-EGFP-DmGIGYF_641-1574_AH

    Plasmid

    #148920

    Purpose

    Insect Expression of DmGIGYF_641-1574

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_641-1574 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmGIGYF_1-640_AH

    Plasmid

    #148919

    Purpose

    Insect Expression of DmGIGYF_1-640

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_1-640 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBacPAK p110a RBD DM

    Plasmid

    #55723

    Purpose

    His3 GST tagged RBD double mutant p110 alpha T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    His3 GST

    Expression

    Mutation

    T207D, K227A

    Promoter

    polyhedrin promoter

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pGL2-100bp

    Plasmid

    #16294

    Depositor

    Wafik El-Deiry

    Article

    Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

    Insert

    p53 promoter fragment (TP53 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    fragment -88 to +12 relative to first transcripti…

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJAF211

    Plasmid

    #52407

    Purpose

    Expresses Flag-tagged mouse Arf6

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

    Insert

    Arf6 (Arf6 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pJH3456

    Plasmid

    #179581

    Purpose

    Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    BFP

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1

    Available since

    March 2, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGG184

    Plasmid

    #165604

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    'CAGCG' PAM replacing the 'CGGCG&#…

    Promoter

    lac

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSicoR human Dicer3

    Plasmid

    #14765

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Dicer shRNA (DICER1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

26. sgTrack-Gateway

    Plasmid

    #114011

    Purpose

    3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL2-50bp

    Plasmid

    #16295

    Depositor

    Wafik El-Deiry

    Article

    Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

    Insert

    p53 promoter fragment (TP53 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    fragment -38 to +12 relative to first transcripti…

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. plexm-mouse LRRC33

    Plasmid

    #111601

    Purpose

    Express mouse LRRC33 in 293T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    mouse LRRC33 (Nrros Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starts at aa 18

    Promoter

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

    Plasmid

    #62945

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

    Plasmid

    #136646

    Purpose

    Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDendra2-Hygro

    Plasmid

    #202407

    Purpose

    Dendra2 expression plasmid

    Depositor

    Morito Kurata

    Article

    Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

    Insert

    Hygromycin
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5FRT/TO-p97-EQ-mycStrep

    Plasmid

    #31838

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    E578Q

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pKG209

    Plasmid

    #45109

    Depositor

    Iain Cheeseman

    Article

    Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

    Insert

    CENP-T (aa1-374) Laci-NLS (CENPT Human)
    

    Use

    Tags

    GFP, Laci-NLS, s peptide, and tev

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMRXIP-Myc-SNAP29

    Plasmid

    #116944

    Purpose

    Expressing Myc-SNAP29 for mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

    Insert

    synaptosomal-associated protein, 29kDa (SNAP29 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEP4L-Erk1

    Plasmid

    #39225

    Depositor

    Melanie Cobb

    Article

    Frost et al Proc Natl Acad Sci U S A. 1994 Apr 26;91

    Insert

    Erk1 (MAPK3 Human)
    

    Use

    Episomal

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

36. TXNIP-py

    Plasmid

    #200090

    Purpose

    Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    TXNIP (TXNIP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    py motifs mutated (aa331-334 and 375-378)

    Promoter

    CMV

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

    Plasmid

    #62944

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

    Plasmid

    #62946

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENV8(GAAA)-GFPuv

    Plasmid

    #70128

    Purpose

    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge