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pORANGE GFP-CACNA1E KI
Plasmid#131481PurposeEndogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK S6K (E389-deltaCT) neo
Plasmid#58516PurposeLentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorInsertRps6kb1 (Rps6kb1 Rat)
UseLentiviralTagsHAExpressionMammalianMutationT389E, stop codon at amino acid 402PromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV HA TrkC
Plasmid#39979DepositorInsertTrkC (Ntrk3 Rat)
UseTagsBM40 signal sequence and HAExpressionMammalianMutationendogenous signal sequence was replaced with BM40…PromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiviral shRNA GEF-H1
Plasmid#21477DepositorInsertrho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a] mut
Plasmid#26576DepositorInsertCACNA1D (Cacna1d Rat)
UseTags6XHis and V5ExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8m
Plasmid#176760PurposeAAV-mediated expression of membrane bound GCaMP8m under the control of GfaABC1D promoterDepositorHas ServiceAAV1 and AAV9Insertlck-jGCaMP8m
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-oA ONE-GO
Plasmid#204330PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-oA ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Lysosomes-20
Plasmid#58022PurposeLocalization: Lysosome Membrane, Excitation: 555, Emission: 584DepositorInsertLAMP1
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJM60-eIF4E
Plasmid#38239DepositorInserteIF4E (Eif4e Rat)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
Myo 1b-myc
Plasmid#135064PurposeExpression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.DepositorInsertMyo 1b with 5' UTR (Myo1b Rat)
UseTagsLinker and MycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-CB2 SH3+
Plasmid#68821PurposeEGFP tagged Collybistin expression in mammalian cellsDepositorInsertCollybistin (Arhgef9 Rat)
UseTagsEGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 141-465
Plasmid#45971DepositorInsertParkin 141-465 (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2 Parkin RBR
Plasmid#31255DepositorInsertParkin (Prkn Rat)
UseTagsGSTExpressionBacterialMutationRBR domain, residues 217-465PromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Evf1
Plasmid#99479PurposeSubcloning of the Evf1 long non-coding RNADepositorInsertEvf1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
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PA-mCherry-miniSOG-Lysosomes-20
Plasmid#57793PurposeLocalization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertLAMP1
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD50E in LAMP1PromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA
Plasmid#51473PurposeBicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6m
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SV2A HaloTag
Plasmid#218545PurposeLentiviral expression of SV2A HaloTagDepositorInsertSV2A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI SV2A pHluorin
Plasmid#218543PurposeMamallian Expression of SV2A pHluorinDepositorInsertSV2A
UseTagsExpressionMammalianMutationS42F, L199PPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8f
Plasmid#167573PurposeDEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.DepositorInsertRibo-GCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
bactin:GFP-LAP2b
Plasmid#105980Purposetransgenesis, ubiquitous expressionDepositorInsertLAP2b
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-EpoR
Plasmid#114289PurposeTET-inducible expression of Erythropoietin receptorDepositorInsertErythropoietin Receptor (Epor Rat)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-ERK2 T183AY185F
Plasmid#118299PurposeExpression of Rat ERK2DepositorInsertERK2 T183AY185F (Mapk1 Rat)
UseTagsGFPExpressionMammalianMutationT183AY185FPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
alpha-0 CFP in pcDNA3.1
Plasmid#36396DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsCFPExpressionMammalianMutationCFP inserted into the KpnI-SpeI sites engineered …PromotercmvAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cx43K258stop-msfGFP
Plasmid#69023PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationdelete codons 258-381 and fuse in frame to monome…PromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBa.VAMP2-GFP-SBP
Plasmid#180249PurposeMammalian expression of VAMP2DepositorInsertVAMP2 (Vamp2 Rat)
UseTagsExpressionMammalianMutationWTPromoterChicken Beta actinAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-p47
Plasmid#21268DepositorInsertp47 (Nsfl1c Rat)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LAMP1-mCherry
Plasmid#214274PurposeMammalian expression of LAMP1-mCherry (lysosome marker)DepositorInsertLAMP1-mCherry (Lamp1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-pHuji
Plasmid#107369PurposeSynaptic vesicle protein VAMP2 fused to the red FP pH sensor pHujiDepositorInsertVAMP2 (Vamp2 Rat)
UseTagspHujiExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Lysosomes-20
Plasmid#57397PurposeLocalization: Lysosome Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertLAMP1
UseTagsmEos2ExpressionMammalianMutationD50EPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
RnBarr1-mVenus
Plasmid#137790PurposeVisualization of beta-arrestin1DepositorInsertRat beta-arrestin 1 (Arrb1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SypHluorin 1x (S1x)
Plasmid#37003DepositorInsertSypHluorin (Syp Rat)
UseTagsHAExpressionMammalianMutationOne copy of pHluorin inserted at site 184-185PromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN_mCyRFP2-CREB
Plasmid#137008PurposeAAV backbone, mCyRFP2-CREB donor for R-CREBDepositorInsertCREB1 (Creb1 Rat)
UseAAVTagsmCyRFP2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-Lysosomes-20
Plasmid#55382PurposeLocalization: Lysosome Membrane, Excitation: 433, Emission: 475DepositorInsertLAMP1
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK285 (Stag-3FLAG-Bsr)
Plasmid#72801PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Bsr
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBK-YE2-BE3
Plasmid#85176PurposeExpresses YE2-BE3 in mammalian cellsDepositorInsertYEE-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218865PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR2Q358y
Plasmid#101028Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only
