We narrowed to 24,925 results for: crispr
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Plasmid#191102PurposeLentiviral expression plasmid of PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseCRISPR and LentiviralExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC015 - dLwCas13a-NF
Plasmid#91905PurposeExpresses negative-feedback dLwCas13a for mammalian RNA targeting/imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdLwCas13a
UseCRISPRTagsNLS and eGFP, ZF CCR5TC, KRAB-A, NLSExpressionMammalianMutationR474A and R1046APromoterCAGAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC154-dual-dCas9VP160-sgExpression
Plasmid#48240PurposeDual expression construct expressing both dCas9VP160 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA-Tag, HA-tag, and VP160ExpressionMammalianMutationD10A H840AAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY016 (pcDNA3.1-hLbCpf1)
Plasmid#69988PurposeExpresses humanized LbCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthLbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DS-NMcas
Plasmid#48646PurposeBacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
NM tracrRNA
UseCRISPRPromoterNM tracrRNA promoter and proCAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB-MeCP2
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateExpressionPlantAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianPromoterTight TRE promoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSQT1313
Plasmid#53370Purposemultiplex gRNA expression plasmidDepositorInsertNone
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME-Cas9
Plasmid#64237Purposeexpresses codon optimized Cas9 in zebrafishDepositorInsertcodon optimized Cas9
UseCRISPRAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0046-EF1a-PspCas13b-NES-HIV
Plasmid#103862PurposeExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorPublicationInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-SFFV-dCas9-BFP
Plasmid#46910PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFPDepositorInsertdCas9-BFP fusion
UseCRISPR and LentiviralTags2xNLS, BFP, and HAExpressionMammalianPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
ExpressionBacterialPromoterPcel and trcAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32
Plasmid#63142PurposeExpress sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.DepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRice snoRNA U3 promoter for gRNA/PTG expression a…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingExpressionMammalianPromoterSynapsin and U6Available sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
ExpressionBacterialAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by