We narrowed to 22,324 results for: Sis
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Plasmid#78814PurposeTo overexpress RIPK3 V460P in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationV460PPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGB1-mCRT(P-domain)
Plasmid#83519PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
UseTagsGB1 and HisExpressionBacterialMutationP domain (contains residues 187-283)PromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGB1-mCRT(C-domain)
Plasmid#83518PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
UseTagsGB1 and HisExpressionBacterialMutationC domain (contains residues 340-399)PromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S44A)
Plasmid#24234DepositorInsertBimL (S44A) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged Serine 44 to AlaninePromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS2_HindIII
Plasmid#65666PurposeFLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII siteDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K570A
Plasmid#14102DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys570 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pICSL11061
Plasmid#68255PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 BimL (T56D)
Plasmid#24532DepositorInsertBim L(T56D) (BCL2L11 Human)
UseTagsT7ExpressionMammalianMutationChanged threonine 56 to aspartic acidPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (1-228)
Plasmid#61932PurposeExpression of hUCH37 (1-228) in E.coliDepositorInserthUCH37 (1-228) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationStop codon introduced after residue K228PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D2
Plasmid#61491Purposeluciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-Dm-miR-13b-1
Plasmid#22762DepositorInsertmicro RNA 13b-1 (mir-13b-1 Fly, primary transcript)
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52542PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL G457D
Plasmid#61367PurposeExpresses Human full length RIPK3 with G457Dmutation in the mammalian cellsDepositorInsertFull length human RIP3 with G457D mutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged G457 to DPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL N464D
Plasmid#61369PurposeExpresses Human full length RIPK3 with N464D mutation in the mammalian cellsDepositorInsertFull length human RIP3 with N464D mutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged N464to DPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-His3MX6
Plasmid#53185Purposechromosomal tagging with stable GFPDepositorInsertHIs3MX6 (HIS3 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A)ExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG610
Plasmid#40195DepositorInsertMex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa 1-355; exon 2 recoded to be RNAi-resi…Promoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ERBB4 (R544W)
Plasmid#29540DepositorInsertERBB4 (ERBB4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533A
Plasmid#14100DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545A
Plasmid#14101DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Gln545 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AQ545A
Plasmid#14103DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Gln545 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 BimL (S58A)
Plasmid#24249DepositorInsertBim L(S58A) (BCL2L11 Human)
UseTagsT7ExpressionMammalianMutationChanged serine 58 to alaninePromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19_21 tRNA genes
Plasmid#242430PurposeIn vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.DepositorInsert21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
hAAVS-RPL23a-SNAP
Plasmid#242187PurposeExpress RPL23a-SNAP in mammalian cellsDepositorInsertRPL23a (RPL23A Human)
UseTagsSNAPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
CASTOR1-HA (D304A)
Plasmid#237419PurposeTransient expression of C-terminally HA-tagged human CASTOR1 (D304A mutant)DepositorInsertCASTOR1 (GATSL3 Human)
UseTagsHAExpressionMammalianMutationD304APromoterAvailable sinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmEos3.1-N1 GBP
Plasmid#220492PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos3.1
UseTagsmEos3.1ExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIV-LacZ-SapI
Plasmid#237610PurposeDestination vector for complete TCR libraryDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGTPA-Gm
Plasmid#171527PurposePlasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant geneDepositorInsertCas12k gRNA, transposon donor cassette, gentamycin-resistant gene
UseCRISPRTagsExpressionMutationPromoterConstitutive promoter J23119Available sinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
rAAV-Ef1α-TdTomato-WPRE-polyA
Plasmid#241828PurposeAAV construct for constitutive expression of TdTomato.DepositorInsertTdTomato-WPRE-polyA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30a-GalK-USP-ET64
Plasmid#234662PurposeExpression an ELP-tagged fusion enzyme of GalK and AtUSPDepositorInsertsUseTagsELPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEPHS1CB0027
Plasmid#227570PurposeLevel 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDSDepositorInsert35Sshort_TMV_TkTXSS (T374G/E379D/Q486H/A745P)_35S
UseTagsExpressionPlantMutationT374G / E379D / Q486H / A745P, BsaI sites removed…Promoter35Sshort_TMV (pICH51277)Available sinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1625
Plasmid#239834PurposeOrufIscB-REC E. coli expression for recombinant purificationDepositorInsertOrufIscB-REC
UseTags14xHis-TwinStrep-bdSUMOExpressionBacterialMutationInsertion of REC domain from NbaCas9-1PromoterT7Available sinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-hGSDMC
Plasmid#218937PurposeExpression of N-terminal FLAG tagged human gasdermin CDepositorInserthuman GSDMC (GSDMC Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-hGSDMB
Plasmid#218936PurposeExpression of N-terminal FLAG tagged human gasdermin BDepositorInserthuman GSDMB (GSDMB Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-mGSDMA3
Plasmid#218942PurposeExpression of N-terminal FLAG tagged mouse gasdermin A3DepositorInsertmouse GSDMA3 (Gsdma3 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1742
Plasmid#239838PurposeOrufIscB-KRK mammalian expressionDepositorInsertOrufIscB-KRK
UseTagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationInsertion of REC domain from NbaCas9-1 + E137K/E4…PromoterCMVAvailable sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1505
Plasmid#239830PurposeOrufIscB-REC mammalian expressionDepositorInsertOrufIscB-REC
UseTagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationInsertion of REC domain from NbaCas9-1PromoterCMVAvailable sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS2096
Plasmid#239835PurposeNovaIscB E. coli expression for recombinant purificationDepositorInsertNovaIscB
UseTags14xHis-TwinStrep-bdSUMOExpressionBacterialMutationInsertion of REC domain from NbaCas9-1 and swap o…PromoterT7Available sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMH-SpAsp1
Plasmid#238315PurposeSpAsp1 protein expression in bacteriaDepositorInsertasp1 (asp1 Fission Yeast)
UseTagsHis7-Tag, Strep-tag II, SUMO proteinExpressionBacterialMutationSpAsp1PromoterAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN201
Plasmid#217917Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y715E, Y717E, Y718EPromoterAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN363
Plasmid#217918Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y715E, Y717E, Y718E, N711YPromoterAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN413
Plasmid#217919Purposeexpress E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutations
UseTagshexa-histidine tagExpressionBacterialMutationY715E, Y717E, Y718E, N711YPromoterAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO539
Plasmid#235752PurposeProtein expression of ScVPS30 and ScVPS38DepositorUseTagsExpressionYeastMutationPromoterGAL-TDH3Available sinceJuly 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT5G02100-mKOk
Plasmid#224848PurposePlasmid for expression of AT5G02100.1 coding sequence tagged with mKOk in plantsDepositorInsertAT5G02100.1 (UNE18 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT5G05380-mKOk
Plasmid#224849PurposePlasmid for expression of AT5G05380.1 coding sequence tagged with mKOk in plantsDepositorInsertAT5G05380.1 (PRA1.B3 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT5G16830-mKOk
Plasmid#224851PurposePlasmid for expression of AT5G16830.1 coding sequence tagged with mKOk in plantsDepositorInsertAT5G16830.1 (SYP21 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT5G59420-mKOk
Plasmid#224855PurposePlasmid for expression of AT5G59420.1 coding sequence tagged with mKOk in plantsDepositorInsertAT5G59420.1 (ORP3C Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT5G65020.1-mKOk
Plasmid#224856PurposePlasmid for expression of AT5G65020.1 coding sequence tagged with mKOk in plantsDepositorInsertAT5G65020.1 (ANNAT2 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT5G65020.2-mKOk
Plasmid#224857PurposePlasmid for expression of AT5G65020.2 coding sequence tagged with mKOk in plantsDepositorInsertAT5G65020.2 (ANNAT2 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(b)-AT1G50260-mKOk
Plasmid#224832PurposePlasmid for expression of AT1G50260.1 coding sequence tagged with mKOk in plantsDepositorInsertAT1G50260.1 (NTMC2T5.1 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only