173,273 results
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Plasmid#171238PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
TagsC-terminal FLAG tag with a linkerExpressionBacterialAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
Tags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Asc
Plasmid#74285PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscIDepositorInsertRosa26 5-homology region
UseMouse TargetingAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmal_c5x_RNAse_Inhib
Plasmid#153314PurposeBacterial expression of an RNase inhibitorDepositorInsertRNAse Inhibitor
TagsMaltose Binding ProteinExpressionBacterialAvailable SinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-EBFP2-nuc
Plasmid#36085DepositorTypeEmpty backboneUseLentiviralTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…Available SinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_HyPer7_mito
Plasmid#238968PurposeMammalian neurons HyPer7 expression targeted to the mitochondrial matrixDepositorInsertHyPer7
UseAAVTags3x Cytochrome C Oxidase Subunit VIII presequenseExpressionMammalianPromoterhSyn1Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1
Plasmid#233078PurposeExpression vector for sgRNA-S1b and eCas12f1DepositorInsertU6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianPromoterhU6, CBhAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
HPV-31neo
Plasmid#153283PurposeNeomycin selectable HPV31 genomeDepositorInsertHPV-31 genome with Neo cassette
UseUnspecifiedMutationHindIII and CsiI site mutated from original Neo c…Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
DPYD-FLAG
Plasmid#61616PurposeThe human DPYD was used to study the effect of DPYD expression on EMTDepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Plac-dCas9 (Plac)
Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-Mut-T-Luc
Plasmid#60490PurposeContains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988DepositorInsertHMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
UseLuciferaseTagsLuciferase reporterExpressionMammalianMutationFour point mutations (G->C or A->C) in the …PromoterPartial HMG-CoA synthase promoter (-324/-225) fus…Available SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLU-VIPR
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonExpressionMammalianAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAR-MT-APC
Plasmid#16487DepositorInsertAPC (APC Human)
ExpressionMammalianAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
Myc-E-Syt1
Plasmid#66833PurposeMammalian Expression, Bacterial ExpressionDepositorAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40
Plasmid#100054PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activationDepositorHas ServiceAAV1, AAV2, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationH134R mutation in ChR2PromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14101 aeBlue
Plasmid#239453Purposeexpress aeBlue chromoproteinDepositorInsertaeBlue
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
Tags10xHisExpressionBacterialAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCKanR-Mu-BsaI
Plasmid#79769PurposeTransposon-propagation plasmid for Mu-BsaI modified transposonDepositorInsertMu-BsaI
Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMasterE
Plasmid#165080PurposeEpisomal expression of reprogramming factors.DepositorInsertsUseEpisomalExpressionMammalianAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-NanoLuc
Plasmid#188247PurposeTemplate plasmid to transcribe mRNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseExpressionMammalianPromoterT7Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
ExpressionBacterialPromoterPPSPAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcw107
Plasmid#62511PurposeGateway Expression VectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterPGKAvailable SinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIB-FLAG(N)-RPB1
Plasmid#231722PurposeProtein expression in insect cellsDepositorAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro FLAG SREBP1
Plasmid#32017DepositorInsertSREBP1 (Srebf1 Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationV225MPromoterCMVAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA
Plasmid#183776PurposeAAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertCre
UseAAVMutationNAPromoterCAGAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only