We narrowed to 22,830 results for: Sis

1. SHC003BSD

   Plasmid

   #133300

   Purpose

   The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-attBr-ZsGreen-attP

   Plasmid

   #51265

   Purpose

   Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-ZsGreen-attP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dapp1-PH pET11a

   Plasmid

   #71376

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Dapp1-PH (DAPP1 Human)
   

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 148-273

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pLenti_CMV:Flag-GpNLuc_SV40:PuroR

   Plasmid

   #183037

   Purpose

   Expresses the FLAG-tagged GpNLuc (Green LumiFluor) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   GpNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-GI-ZFP2

   Plasmid

   #42215

   Purpose

   Expresses GI-ZFP2 for light-inducible activation of gene expression

   Depositor

   Charles Gersbach

   Article

   Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

   Insert

   GIGANTEA-FLAG-ZFP2 (GI Mustard Weed, Synthetic)
   

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Jan. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRU61

   Plasmid

   #167692

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::asCpf1
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSuper mouse Dicer3

   Plasmid

   #14778

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Dicer shRNA (Dicer1 Mouse)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1(+)-RABIF-FLAG

   Plasmid

   #135558

   Purpose

   Express human RABIF in mammalian cells, FLAG at N terminal

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   RABIF (RABIF Human)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   T7 promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIAA5::LUC

   Plasmid

   #141169

   Purpose

   Luminescent reporter for auxin signaling

   Depositor

   Vardis Ntoukakis, Patrick Schäfer

   Article

   Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

   Insert

   AtIAA5 promoter
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtIAA5, At1g15580

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gma-pCAMBIA-1301-STTM171

    Plasmid

    #84080

    Purpose

    Disturb the expression of miR171 in soybean

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR171
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mOPN-PB0

    Plasmid

    #106449

    Purpose

    Bacterial expression of murine osteopontin

    Depositor

    Georg Weber

    Article

    Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

    Insert

    osteopontin (Spp1 Mouse)
    

    Use

    Retroviral

    Mutation

    WT

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG425 GAL Pong TPase L418A, L420A

    Plasmid

    #145795

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock, Susan Wessler

    Article

    Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

    Insert

    Pong TPase L418A, L420A
    

    Expression

    Yeast

    Mutation

    L418A, L420A

    Available Since

    Jan. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pB-Hygro-rKIF5B-GFP

    Plasmid

    #210335

    Purpose

    Overexpression of rKIF5B

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    rKIF5B (Kif5b Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMVS-V1

    Plasmid

    #183094

    Purpose

    Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

    Depositor

    Bastian Molitor, Lars Angenent

    Article

    Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

    Inserts

    pME2001
    thermostable neomycin phosphotransferase gene
    

    Use

    Bacterial propagation and conjugative

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-mTUT7

    Plasmid

    #60044

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT7

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT7 (Zcchc6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pPB-EF1a-BCL2-hygro

    Plasmid

    #222581

    Purpose

    PiggyBac transposon plasmid for constitutive expression of BCL2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    BCL2 (BCL2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

    Plasmid

    #211906

    Purpose

    Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

    Depositor

    Yannick Doyon

    Article

    Levesque et al bioRxiv 2023.09.14.557485

    Insert

    DARIC-CD22-BBz
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR L1-vas2-Cre-L4

    Plasmid

    #62301

    Purpose

    Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    Cre recombinase under control of vasa promoter and terminator
    

    Tags

    HA and SV40 NLS

    Expression

    Insect

    Promoter

    vasa

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB6627

    Plasmid

    #106159

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYFAC-riboB

    Plasmid

    #168975

    Purpose

    Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Expression in aspergillus nidu…

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET16b-Antp

    Plasmid

    #86866

    Purpose

    Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

    Depositor

    Michael Chin

    Article

    Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

    Insert

    Linker-Antp
    

    Tags

    Antp

    Expression

    Bacterial

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBS/KS-mId3sp

    Plasmid

    #16045

    Depositor

    Robert Benezra

    Article

    Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

    Insert

    Id3 (Id3 Mouse)
    

    Expression

    Bacterial

    Mutation

    cDNA nucleotides 365-635

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

23. FUW-mTurquoise

    Plasmid

    #193092

    Purpose

    constitutive expression of mTurquoise in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    mTurquoise
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. WFS1 P724L-Flag-pcDNA3

    Plasmid

    #13012

    Depositor

    Fumihiko Urano

    Article

    Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

    Insert

    Wolfram Syndrome 1 (WFS1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed P 724 to L

    Available Since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pUAST cyto roGFP2-Orp1

    Plasmid

    #64996

    Purpose

    for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGRCRE

    Plasmid

    #129116

    Purpose

    Expresses cre recombinase in Z. rouxii yeast cells

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Insert

    cre
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    S. cerevisiae GAL1 promoter

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMaCTag-P25

    Plasmid

    #120036

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCfB6638

    Plasmid

    #106165

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti_CMV:Flag-OgNLuc_SV40:PuroR

    Plasmid

    #183038

    Purpose

    Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    OgNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-GFP-MTn-KDEL

    Plasmid

    #154348

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCA-G-intron-G

    Plasmid

    #40027

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP exon 1
    GFP exon 2
    beta-globin intron
    

    Expression

    Mammalian

    Mutation

    insertion of a loxP site, nucleotides 1-274, and …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMaCTag-Z11

    Plasmid

    #120054

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet-i
    

    Use

    Pcr template

    Tags

    mScarlet-i

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKPY197

    Plasmid

    #62599

    Purpose

    Encodes Thr412Gly-CePheRS Alpha Subunit

    Depositor

    David Tirrell

    Article

    Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

    Insert

    Thr412Gly-CePheRS Alpha Subunit
    

    Tags

    HIS

    Expression

    Worm

    Mutation

    Thr412Gly

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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34. pGENE:EFF-1::mCherry::V5

    Plasmid

    #133020

    Purpose

    EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

    Insert

    EFF-1::mCherry::V5
    

    Tags

    V5; mCherry

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pV1539

    Plasmid

    #111434

    Purpose

    Genedrive entry vector for Candida albicans

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 3'-3xFLAG minimal CMV pCDNA5

    Plasmid

    #212045

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Abp1-tDimerRFP

    Plasmid

    #27699

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Abp1 (Dbnl Mouse)
    

    Tags

    tDimer-RFP

    Expression

    Mammalian

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pCMV-FLAG TIF1gamma M (456-887)

    Plasmid

    #15735

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    TIF1 gamma M (TRIM33 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Middle region (amino acids 456-887).

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCS2+/N-SNAPf

    Plasmid

    #184415

    Purpose

    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2+/C-SNAPf

    Plasmid

    #184416

    Purpose

    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge