166,224 results

1. LentiCRISPRv2GFP

   Plasmid

   #82416

   Purpose

   3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

   Depositor

   David Feldser

   Article

   Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS (P2A)

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIF1079

   Plasmid

   #237445

   Purpose

   Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

   Depositor

   IIya Finkelstein

   Article

   Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

   Inserts

   SpCas9
   Efe1-RT
   EMX1 sgRNA
   Efe1-ncRNA targeting EMX1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CAG, H1, and U6

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pV238-vPE

   Plasmid

   #225258

   Purpose

   Mammalian expression of vPE

   Depositor

   Vikash Chauhan, Robert Langer, Phil Sharp

   Article

   Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

   Insert

   vPE
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R221K K848A H982A N1317R

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH47742::p35S-PE2max-NC::tEURb7

   Plasmid

   #227693

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLSL.R.Ly

   Plasmid

   #227697

   Purpose

   Reengineered geminiviral replicon vector

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. mKeima-Red-Mito-7

   Plasmid

   #56018

   Purpose

   Localization: Mitochondria, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Tags

   mKeima-Red

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HA

   Plasmid

   #128034

   Purpose

   Mammalian expression vector with N terminal HA tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLCAR-CD19-28z

    Plasmid

    #135991

    Purpose

    Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFV-Helper1

    Plasmid

    #92073

    Purpose

    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Insert

    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    

    Use

    Helper plasmid, for run-off transcription and rna…

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xHA-TurboID-NLS_pCDNA3

    Plasmid

    #107171

    Purpose

    expresses 3xHA-tagged TurboID in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pI3_EGFP

    Plasmid

    #239335

    Purpose

    Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP- SEC61B

    Plasmid

    #121159

    Purpose

    Mammalian expression of GFP tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-IP-HaloTag7-LC3

    Plasmid

    #184899

    Purpose

    Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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