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Showing: 1101 - 1150 of 2333 results

  10. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid
    #110555
    Purpose
    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.
    Depositor
    Martin Pule
    Article
    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg
    Insert
    Anti-RD114 heavy chain
    Use
    Tags
    mIgG2A
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF-1 alpha core promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110555-plasmid-map-sequence-id-212943

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  13. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

    Plasmid
    #110556
    Purpose
    pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.
    Depositor
    Martin Pule
    Article
    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg
    Insert
    anit-RD114 light chain
    Use
    Tags
    mKappa
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF-1 alpha core promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110556-plasmid-map-sequence-id-273295

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  14. LSB-hsa-miR-941

    Plasmid
    #103757
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-941 target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103757-plasmid-map-sequence-id-196562

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  32. SpCas9-NG-miniCGBE1

    Plasmid
    #198538
    Purpose
    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198538-plasmid-map-sequence-id-388888

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  35. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid
    #141237
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    GCaMP6f
    Use
    AAV and Cre/Lox
    Tags
    6XHIS and Xpress
    Expression
    Mutation
    Promoter
    Human elongation factor-1 alpha (EF-1 alpha)
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    141237-plasmid-map-sequence-id-431695

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  36. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214474-plasmid-map-sequence-id-425600

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  42. LSB-hsa-miR-1

    Plasmid
    #103160
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-1 target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103160-plasmid-map-sequence-id-213255

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  49. LSB-hsa-let-7a-5p

    Plasmid
    #103146
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7a-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103146-plasmid-map-sequence-id-213119

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Showing: 1101 - 1150 of 2333 results