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pLKO mouse shRNA 2 DEPTOR

Plasmid

#21338

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR shRNA 2 (Deptor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

Plasmid

#173963

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif

Use

Luciferase

Tags

Expression

Mutation

1 mutated Coordinator motif

Promoter

SV40 promoter

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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V622 pGEX-2TK-LP - Acceptor

Plasmid

#11716

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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MSGV Rh Acceptor PGK-NGFR

Plasmid

#64272

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1 Insulator ObLiGaRe/EPB71

Plasmid

#90018

Purpose

Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

Depositor

Eric-Paul Bennett

Article

Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

Insert

MCS flanked by insulator and inverted ZFN binding sites

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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retro-gfpIkkb-puro vector

Plasmid

#58251

Purpose

Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Ikkβ (Ikbkb Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
hSTARR-seq_OCT4 3UTR vector

Plasmid

#99295

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC6-Flag CASTOR1 I280A

Plasmid

#84497

Purpose

Lentiviral expression of CASTOR2 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

sestrin2

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
V1662 pLP-RFP - Acceptor

Plasmid

#11700

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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V1663 pLPS-3'RFP - Acceptor

Plasmid

#11701

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR221-ACE2 receptor 19-615

Plasmid

#167012

Purpose

Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 19-615, No stop codon

Promoter

Available since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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V518 pLPS-3'dMyc - Acceptor

Plasmid

#11714

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

2xMyc

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V956 pLPS-3'ECFP - Acceptor

Plasmid

#11698

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V517 pLP-dMyc SD - Acceptor

Plasmid

#11713

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

2xMyc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-Sa sgRNA expression vector

Plasmid

#107720

Purpose

For in vitro transcription of Sa sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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p53 S23A targeting vector

Plasmid

#12167

Depositor

Tyler Jacks

Article

MacPherson et al EMBO J. 2004 Sep 15. 23(18):3689-99.

Insert

p53 (Trp53 Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Ser23 mutation (S23A) in exon 2 and puromycin gen…

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
R777-E302 Hs.RPTOR-nostop

Plasmid

#70586

Purpose

Gateway ORF clone of human RPTOR [NM_020761.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

RPTOR (RPTOR Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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