We narrowed to 23,941 results for: nes

1. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

   Plasmid

   #71396

   Purpose

   2Gi2R assay

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Inserts

   2xGFP:NES
   2xRFP:NLS
   

   Use

   Lentiviral

   Tags

   IRES

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha and IRES

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

   Plasmid

   #101060

   Purpose

   AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

   Plasmid

   #174081

   Purpose

   Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
   

   Use

   Tags

   NES-Flag

   Expression

   Mammalian

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   CMV

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-mCherry-NES reporter (pDN160)

   Plasmid

   #72660

   Purpose

   NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Insert

   NLS-mCherry-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200406

   Purpose

   Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   MCP-ADAR2dd(E488Q)-NES
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   E488Q (hyperactive)

   Promoter

   CMV

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0045-EF1a-PguCas13b-NES-HIV

   Plasmid

   #103861

   Purpose

   Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PguCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0044-EF1a-RanCas13b-NES-mapk

   Plasmid

   #103855

   Purpose

   Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   RanCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and mapk NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV:: NES-FKBP-iLID-LRP6c

    Plasmid

    #221774

    Purpose

    Expresses NES-FKBP-iLID-LRP6c component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

    Insert

    pCMV:: NES-FKBP-iLID-LRP6c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LTR5HS S. pyogenes scaffold CARGO array

    Plasmid

    #191316

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP

    Plasmid

    #194696

    Purpose

    HuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafish

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Use

    Zebrafish expression

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    elavl3(HuC)

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. nontarget S. pyogenes scaffold CARGO array

    Plasmid

    #191319

    Purpose

    Contains an array of nontargeting guide RNAs

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG1280 NLS-mCherry-FIREtag-NES

    Plasmid

    #205172

    Purpose

    expresses NLS-mCherry-FIREtag-NES in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NLS-mCherry-FIREtag-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-NES-NIR-GECO1

    Plasmid

    #113683

    Purpose

    Express NIR-GECO1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NES-NIR-GECO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-hSyn1-NES-ChemoG-CaM

    Plasmid

    #193817

    Purpose

    Expression of ChemoG-CaM in primary neurons

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG-CaM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    EGFP:A206K; HT7: L271E

    Promoter

    hSyn1

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid

    #127358

    Purpose

    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-NES-DEVD-mCardinal-NLS

    Plasmid

    #164052

    Purpose

    Fluorescent reporter for caspase-3

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-mCardinal-NLS
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+_Flag-myc-NES-Tir1

    Plasmid

    #117717

    Purpose

    mRNA synthesis of Flag-myc-NES-TIR1 for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    Flag-Myc-NES

    Expression

    Mammalian

    Mutation

    codon optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNES-EGFP-C1-flex-PABD-Spo20

    Plasmid

    #220081

    Purpose

    Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Use

    Tags

    X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

    Expression

    Mammalian

    Mutation

    amino acids 51-91

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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