We narrowed to 22,832 results for: Sis

1. pPV230.13

   Plasmid

   #14902

   Depositor

   James Lok

   Article

   Li et al Int J Parasitol. 2006 May 31. 36(6):671-

   Insert

   Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region
   

   Expression

   Worm

   Mutation

   Ss era-1 promoter region as a HindIII-PstI fragme…

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pV1386

   Plasmid

   #111445

   Purpose

   Solo vector pV1382 + sgScADE2

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2/stop-codon repair
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-SERTG56A -myc

   Plasmid

   #107459

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Expression

   Mammalian

   Mutation

   G56A

   Promoter

   CMV

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TrkA-mCherry-CRY2

   Plasmid

   #106167

   Purpose

   mammalian expression of full length TrkA fused to mCherry and CRY2PHR (aa 1-498)

   Depositor

   Bianxiao Cui

   Article

   Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

   Insert

   TrkA mCherry CRY2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSR-puro-Sec5

   Plasmid

   #31117

   Depositor

   Christopher Counter

   Article

   Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

   Insert

   Sec5 shRNA (EXOC2 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-myc-FRNK

   Plasmid

   #74481

   Purpose

   This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).

   Depositor

   J Thomas Parsons

   Article

   Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.

   Insert

   FRNK (PTK2 Chicken)
   

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   FRNK is the C-terminal, noncatalytic domain of th…

   Promoter

   CMV

   Available Since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pMaCTag-P04

   Plasmid

   #120015

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mTFP1
   

   Use

   Pcr template

   Tags

   mTFP1

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJP102

   Plasmid

   #176480

   Purpose

   Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Inserts

   actin > NatR
   EF1 > mScarlet
   

   Use

   Capsaspora owczarzaki expression

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJP103

   Plasmid

   #176481

   Purpose

   Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Inserts

   actin > HygR
   EF1 > mScarlet
   

   Use

   Capsaspora owczarzaki expression

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TA0474_pET21a(+)

    Plasmid

    #13839

    Depositor

    SeWon Suh

    Article

    Kang et al Acta Crystallograph Sect F Struct Biol C

    Insert

    IS200 transposase (Ta0474 Thermoplasma acidophilum)
    

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Available Since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPTXLUC

    Plasmid

    #68504

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Promoter

    OPTX

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPKm-145

    Plasmid

    #90505

    Purpose

    Empty plasmid, pSIN-EF1-alpha-IRES-puro

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBSHSP:FLP;CMV:tdTomato-SceI

    Plasmid

    #30523

    Depositor

    Gerhart Ryffel

    Article

    Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

    Insert

    FLP recombinase tdTomato
    

    Use

    Flp/frt, heat shock inducible, i-scei megabase ve…

    Mutation

    R415H

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ150-cul4

    Plasmid

    #80353

    Purpose

    Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::cul-4 3'UTR
    

    Expression

    Worm

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2-cryR;mafbbCE1-P1Egfp

    Plasmid

    #90155

    Purpose

    contains mafbb enhancer sequence, and mafbb specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    mafbbE1P1Egfp
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRUSH

    Plasmid

    #54479

    Purpose

    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

    Depositor

    Laurie Jackson-Grusby

    Article

    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and RNAi

    Tags

    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-FLAG/MKRN1(1-369)

    Plasmid

    #78753

    Purpose

    To overexpress MKRN1(1-369) in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1-369

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE658

    Plasmid

    #153256

    Purpose

    M3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-mCherry-Emi1_control UTRs

    Plasmid

    #67925

    Purpose

    Expresses mCherry-Emi1 with control UTRs in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    mCherry-Emi1 (FBXO5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ad-shMKP-3

    Plasmid

    #131530

    Purpose

    Silence the MKP-3 expression

    Depositor

    Haiyan Xu

    Article

    Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

    Insert

    Short hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pETM6-At4CL-PhCHS-MsCHI

    Plasmid

    #73392

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    MsCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. plentiCRISPR-sgMDH1

    Plasmid

    #72875

    Purpose

    The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgMDH1
    

    Use

    Lentiviral

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pGFP-Ub-VPS13D_dN

    Plasmid

    #176465

    Purpose

    express VPS13D lacking N-terminal 117 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Tags

    GFP-Ub

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBGT

    Plasmid

    #62667

    Purpose

    Donor vector for iRFP knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iRFP
    

    Use

    Cre/Lox

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLLX8

    Plasmid

    #79838

    Purpose

    Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

    Depositor

    Cammie Lesser, Stephen Lory

    Article

    Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Type

    Empty backbone

    Use

    Contains yeast counter selection gene, cyh2 and b…

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FT1

    Plasmid

    #31435

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Fused Toes Homolog (AKTIP Human)
    

    Tags

    HA

    Expression

    Yeast

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pDDGFP-Leu2d_GgPCFT

    Plasmid

    #165414

    Purpose

    expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT (SLC46A1 Chicken, Synthetic)
    

    Tags

    GFP-His8

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. p6721 MSCV-IP N FlagHA 16E6 8S9A10T

    Plasmid

    #44153

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    E6 8S9A10T mutant

    Promoter

    MSCV LTR

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3-FLAG-mTUT1

    Plasmid

    #60038

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT1

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT1 (Mtpap Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pMaCTag-Z18

    Plasmid

    #120061

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p6722 MSCV-IP N FlagHA 16E6 I128T

    Plasmid

    #44154

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    HPV16 E6 I128T mutant

    Promoter

    MSCV LTR

    Available Since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

32. 3445 pcDNA3 Bid p15

    Plasmid

    #8812

    Depositor

    Stanley Korsmeyer

    Article

    Zha et al Science 2000 Dec 1;290(5497):1761-5.

    Insert

    Bid p15 (Bid Mouse)
    

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

33. nPEX-LV

    Plasmid

    #12119

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Matrix Metalloproteinase 2 (Mmp2 Mouse)
    

    Use

    Lentiviral

    Mutation

    noncatalytic fragment of MMP2 amplified via PCR, …

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pBDT

    Plasmid

    #62660

    Purpose

    Targeting vector for PITT landing pad knock-in into the Rosa26 locus

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Inserts

    eGFP
    neomycin
    

    Use

    Mouse Targeting

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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35. pCGN-ELK-1 R mut

    Plasmid

    #27159

    Purpose

    For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R box mutant: K249A/V250A/E251A

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

36. RCAS (A) Hoxd13 (CT#441)

    Plasmid

    #13985

    Depositor

    Cliff Tabin

    Article

    Goff et al Development. 1997 Feb . 124(3):627-36.

    Insert

    Hoxd-13 (HOXD13 Chicken)
    

    Use

    Retroviral; Avian expression

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pCMV-ABEmax(TadA E59A, TadA*E59A)

    Plasmid

    #125649

    Purpose

    pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

    Insert

    ABEmax(TadA E59A TadA* E59A)
    

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRU205

    Plasmid

    #167685

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCpf1
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRU321

    Plasmid

    #167681

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::asCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRU320

    Plasmid

    #167682

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge