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167,150 results

Showing: 9281 - 9320 of 167150 results

  4. pSLIK_Hyg_mTurquoise2_Atg4BC74A

    Plasmid
    #161733
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available Since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161733-plasmid-map-sequence-id-318425

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  9. pCMV_53BP1_FLAG

    Plasmid
    #237345
    Purpose
    transient overexpression in mammalian cells
    Depositor
    Michele Pagano
    Insert
    53BP1 (TP53BP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    237345-plasmid-map-sequence-id-473142

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  11. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Article
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    12X His tag, 3XNLS, FLAG, and MBP
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

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  16. Mito-GA

    Plasmid
    #209865
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209865-plasmid-map-sequence-id-415469

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  19. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

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  24. TFORF0098

    Plasmid
    #142460
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MSGN1 (MSGN1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142460-plasmid-map-sequence-id-391606

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  34. mCherry-Ezrin-C-14

    Plasmid
    #55042
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    NM_003379.4 (EZR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pL99

    Plasmid
    #78577
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Expression
    Bacterial
    Promoter
    inducible PnitA-NitR system
    Available Since
    May 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    78577-plasmid-map-sequence-id-263121

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  37. pCMV-Spike-VLP

    Plasmid
    #173922
    Purpose
    Expresses virus-like particles based upon the original SARS-Cov-2
    Depositor
    Andreas Stuermer
    Inserts
    SARS-2 Spike Protein (S SARS-COV-2, Synthetic, Human)
    SARS-Cov-2 E protein (E SARS-COV-2, Synthetic, Human)
    SARS-Cov-2 M Protein (M SARS-COV-2, Synthetic, Human)
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Promoter
    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…
    Available Since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173922-plasmid-map-sequence-id-358853

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Showing: 9281 - 9320 of 167150 results