We narrowed to 43,346 results for: cha;

1. pRSFDUET-1_VNp-anti-Lysozyme fAb

   Plasmid

   #214951

   Purpose

   Bacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-Lysozyme Fab heavy chain fusion
   VNp-anti-Lysozyme Fab light chain fusion
   

   Use

   Tags

   VNp

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MZB215

   Plasmid

   #221767

   Purpose

   Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf6 (ARF6 Human)
   

   Use

   Tags

   CPD-10xHis

   Expression

   Bacterial

   Mutation

   Q67L

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MZB218

   Plasmid

   #221769

   Purpose

   Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf4 (ARF4 Human)
   

   Use

   Tags

   CPD-10xHis

   Expression

   Bacterial

   Mutation

   Q71L

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MZB219

   Plasmid

   #221770

   Purpose

   Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf5 (ARF5 Human)
   

   Use

   Tags

   CPD-10xHis

   Expression

   Bacterial

   Mutation

   Q71L

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-GST-2

   Plasmid

   #228984

   Purpose

   For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-2
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21-pelB-GST-7

   Plasmid

   #228989

   Purpose

   For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21-pelB-LaTdT-38

   Plasmid

   #228979

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-38
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-GST-1

   Plasmid

   #228983

   Purpose

   For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21-pelB-LaTdT-44

   Plasmid

   #228981

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-44
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaTdT-45

    Plasmid

    #228982

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-45
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-GST-8

    Plasmid

    #228990

    Purpose

    For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-8
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-GST-5

    Plasmid

    #228987

    Purpose

    For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-GST-6

    Plasmid

    #228988

    Purpose

    For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-6
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWR58

    Plasmid

    #202795

    Purpose

    integrating bioluminescene reporter for Cas9 activity in S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEMT-mMyh13CDS

    Plasmid

    #192853

    Purpose

    Plasmid to produce ISH probe for mMyh13 gene with CDS sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 13 (Myh13 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEMT-mMyh13UTR

    Plasmid

    #192854

    Purpose

    Plasmid to produce ISH probe for mMyh13 gene with UTR sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 13 (Myh13 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPICZ A-Strep-Opa1 (SB251)

    Plasmid

    #227600

    Purpose

    Inducible expression of Strep-tagged Opa1 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Tags

    Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 253-960 only

    Promoter

    AOX1

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225645

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tetA and LacO3O1 promoters

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225648

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    LacO3O1 and tetA promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225647

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    LacO3O1 and tetA promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225646

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tetA and LacO3O1 promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a- mCherry-FIVAR-HIS-ybbR

    Plasmid

    #169138

    Purpose

    E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    mCherry
    

    Use

    Tags

    6x Histag, FIVAR tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Pt NaV1.4

    Plasmid

    #200233

    Purpose

    Expression in mammalian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

    Insert

    Phyllobates terriblis NaV1.4 – muscle channel
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Dt NaV1.4

    Plasmid

    #200250

    Purpose

    Expression in mammalian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

    Insert

    Dendrobates tinctorius NaV1.4 – muscle channel
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDUP2

    Plasmid

    #223517

    Purpose

    cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP (second copy)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RcSxph E540A

    Plasmid

    #194019

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RcSxph E540D

    Plasmid

    #194020

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540D

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RcSxph E540Q

    Plasmid

    #194021

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540Q

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RcSxph Y558A

    Plasmid

    #194022

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RcSxph Y558F

    Plasmid

    #194023

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558F

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. RcSxph Y558I

    Plasmid

    #194024

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558I

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RcSxph F561A

    Plasmid

    #194025

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    F561A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RcSxph T563A

    Plasmid

    #194026

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    T563A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RcSxph P727A

    Plasmid

    #194027

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    P727A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RcSxph I782A

    Plasmid

    #194028

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    I782A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RcSxph I782F

    Plasmid

    #194029

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    I782F

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RcSxph F784A

    Plasmid

    #194030

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    F784A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RcSxph F784C

    Plasmid

    #194031

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    F784C

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RcSxph F784L

    Plasmid

    #194032

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    F784L

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. RcSxph F784S

    Plasmid

    #194033

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    F784S

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge