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We narrowed to 30,195 results for: REP

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Showing: 4681 - 4700 of 30195 results

  3. pLCKO2

    Plasmid
    #125518
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125518-plasmid-map-sequence-id-248252

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  12. pVC-FUS-WT

    Plasmid
    #175066
    Purpose
    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175066-plasmid-map-sequence-id-372335

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  14. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  16. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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Showing: 4681 - 4700 of 30195 results