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Showing: 11701 - 11750 of 14587 results

  1. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available Since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pGBW-m4046763

    Plasmid
    #145606
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145606-plasmid-map-sequence-id-298330

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  16. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  20. pCG-D-mSpyCatcher-His6-E

    Plasmid
    #87376
    Purpose
    An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    mSpyCatcher
    Use
    Synthetic Biology
    Tags
    His6 tag
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87376-plasmid-map-sequence-id-183395

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  23. pGBW-m4046764

    Plasmid
    #145673
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145673-plasmid-map-sequence-id-292487

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  25. pSecEffector

    Plasmid
    #172891
    Purpose
    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901
    Depositor
    Urartu Seker
    Article
    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696
    Inserts
    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    Expression
    Bacterial
    Promoter
    ProD, nTETO, and pBAD /AraC
    Available Since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    172891-plasmid-map-sequence-id-368224

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  29. pGBW-m4046854

    Plasmid
    #145759
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145759-plasmid-map-sequence-id-297847

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  34. pGBW-m4046832

    Plasmid
    #145691
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145691-plasmid-map-sequence-id-298369

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  39. pTd-4-15-13-10

    Plasmid
    #63835
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  43. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available Since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

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  44. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid
    #168207
    Purpose
    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection
    Depositor
    Markus Hafner
    Article
    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.
    Type
    Empty backbone
    Use
    Unspecified; Pdest gateway
    Tags
    V5 APEX2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168207-plasmid-map-sequence-id-331104

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  47. pTC-CMV-Tet

    Plasmid
    #85577
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liver
    Depositor
    Ursula Ehmer
    Article
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Insert
    CreER
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    ApoE.HCR.hAAT
    Available Since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85577-plasmid-map-sequence-id-169840

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Showing: 11701 - 11750 of 14587 results