Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

164,127 results

Showing: 9441 - 9480 of 164127 results

  1. EBFP2-Nucleus-7

    Plasmid
    #55249
    Purpose
    Localization: Nuclear Localization, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    EBFP2-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pUltra-Plus-H2B-mTagBFP2

    Plasmid
    #161790
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Bicistronic
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC and UbC promoter
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161790-plasmid-map-sequence-id-316820

    Enlarge

  3. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

    Enlarge

  6. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

    Enlarge

  12. DVK_pBR322_AE

    Plasmid
    #217592
    Purpose
    Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    217592-plasmid-map-sequence-id-482298

    Enlarge

  17. Sc RFC5-pCDFDuet

    Plasmid
    #239202
    Purpose
    Overexpress Sc RFC5 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    RFC5 (RFC5 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239202-plasmid-map-sequence-id-474606

    Enlarge

  18. 8061-E31

    Plasmid
    #234268
    Purpose
    Gateway ORF Entry clone of mouse Smad1 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Smad1 (Smad1 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234268-plasmid-map-sequence-id-468855

    Enlarge

  20. pAIP4

    Plasmid
    #233686
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.
    Depositor
    Christopher Bahl
    Insert
    Smt3 (SMT3 )
    Use
    Tags
    HisTag
    Expression
    Mutation
    Promoter
    T7
    Available Since
    April 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233686-plasmid-map-sequence-id-468017

    Enlarge

  21. pEW108

    Plasmid
    #232823
    Purpose
    Expresses yeast compass complex in insect cells
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    Set1(762-1080)
    Promoter
    polyhedrin promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232823-plasmid-map-sequence-id-465728

    Enlarge

  22. pAIP4_9C74

    Plasmid
    #234238
    Purpose
    Heterologous protein expression of variant of super folded GFP in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    9C74
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234238-plasmid-map-sequence-id-464202

    Enlarge

  23. SaDHFR-pNIC-Bsa4

    Plasmid
    #231039
    Purpose
    Expresses Staphylococcus aureus Dihydrofolate reductase under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    SaDHFR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231039-plasmid-map-sequence-id-462635

    Enlarge

  26. 18065-M35-412

    Plasmid
    #225670
    Purpose
    Lentiviral protein expression of a TGF-beta pathway reporter
    Depositor
    Dominic Esposito
    Insert
    dsEGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    S3x6-JunBp
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225670-plasmid-map-sequence-id-452343

    Enlarge

  34. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid
    #169524
    Purpose
    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169524-plasmid-map-sequence-id-364607

    Enlarge

  37. TFORF1217

    Plasmid
    #142757
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRRX1 (PRRX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142757-plasmid-map-sequence-id-391466

    Enlarge

  38. EF1a_Puro_Telo_v2

    Plasmid
    #195139
    Purpose
    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction
    Depositor
    Jason Sheltzer
    Article
    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11
    Insert
    Telomere (RTEL1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195139-plasmid-map-sequence-id-442720

    Enlarge

Showing: 9441 - 9480 of 164127 results