161,805 results

1. pdRBFC-NS1-YC

   Plasmid

   #74724

   Purpose

   Express C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

   Depositor

   Article

   Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

   Insert

   Influenza A virus NS1 protein, partial
   

   Use

   Double-stranded rna labeling

   Tags

   FLAG and YFP C-terminal half domain

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbE8a-RFP

   Plasmid

   #35268

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BAD

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hBACCS2-IRES-mCherry

   Plasmid

   #72892

   Purpose

   Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   hBACCS2-IRES-mCherry
   

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TagBFP2-PIP4K2B

   Plasmid

   #202728

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:PIP4K2B (PIP4K2B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB_TRE3G_scFV-sfGFP-G9aCD

   Plasmid

   #235580

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   G9a (Ehmt2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVTNT-T7-KPNA4

   Plasmid

   #26680

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 4 (KPNA4 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1-SypHer3s

   Plasmid

   #108118

   Purpose

   Ratiometric pH probe with enhanced brightness for mammalian cells expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV.CamKII.GCaMP6s.WPRE.SV40

   Plasmid

   #107790

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

   Depositor

   James M. Wilson

   Has Service

   AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-GFAP-EGFP

   Plasmid

   #50473

   Purpose

   EGFP under the control of GFAP promoter

   Depositor

   Bryan Roth

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-kanMX6-P3nmt1-GFP

    Plasmid

    #39289

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE

    Plasmid

    #183528

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    rsChRmine-oScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I146M/G174S

    Promoter

    Ef1a

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPR-StAR4BN (Lenti)

    Plasmid

    #222692

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

    Plasmid

    #197989

    Purpose

    For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

    Depositor

    Bo Wang

    Article

    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

    Insert

    HSALNT0217290
    

    Use

    Lentiviral

    Tags

    lac

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-p38alpha

    Plasmid

    #86832

    Purpose

    mammalian expression of GFP-p38alpha

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38alpha (MAPK14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTn7-SCOUT11.G

    Plasmid

    #200999

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 3xFlag IFIT3

    Plasmid

    #53553

    Purpose

    mammalian expression of IFIT3

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT3 (IFIT3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. MYD88

    Plasmid

    #13092

    Depositor

    Ruslan Medzhitov

    Insert

    MYD88 (Myd88 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Use

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-BHMT-I/G

    Plasmid

    #104442

    Purpose

    Autophagy cargo assay

    Depositor

    Carol Mercer

    Article

    Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

    Insert

    BHMT (BHMT Human)
    

    Use

    Tags

    GFP-myc GFP is from Aequorea victoria and GST

    Expression

    Mammalian

    Mutation

    N43A in GST (in a potential KFERQ-like sequence).…

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV2-SEP-GluA1 (M1)

    Plasmid

    #64942

    Purpose

    mammalian expression of rat GluA1

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    GluA1 (Gria1 Rat)
    

    Use

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-SynB

    Plasmid

    #174861

    Purpose

    Expresses mouse syncytin B in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    syncytin B (Synb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOpen-TEV protease

    Plasmid

    #165509

    Purpose

    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    TEV protease
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. CRISPR_HLA_class_I_2

    Plasmid

    #164987

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-Neo-Bam p53 wt

    Plasmid

    #16434

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTn7-SCOUT11.R

    Plasmid

    #201003

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-mCherry, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-hA3A-BE3

    Plasmid

    #113410

    Purpose

    Expresses hA3A-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3 (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV Flag ATRwt

    Plasmid

    #41909

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 2001 Nov 23;294(5547):1713-6.

    Insert

    ATR ATR serine/threonine kinase (ATR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SRa

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYLCRISPR/Cas9P35S-N

    Plasmid

    #66191

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ITPKA-mNeonGreen

    Plasmid

    #98883

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    ITPKA
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmiRA1-MYB

    Plasmid

    #141120

    Purpose

    Lentiviral overexpression of MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCI-EGFP-NR1 wt

    Plasmid

    #45446

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pLKO.1 hygro shRNA E-cadherin

    Plasmid

    #18802

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.0-Magneto2.0-p2A-mCherry

    Plasmid

    #74308

    Purpose

    Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
    

    Use

    Tags

    Flag and p2A-mCherry

    Expression

    Mammalian

    Mutation

    TRPV4: delta 760–871

    Promoter

    CMV

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mTagBFP2-Vimentin-7

    Plasmid

    #55333

    Purpose

    Localization: Intermediate Filaments, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

39. CMV-G-GECO1.2

    Plasmid

    #32446

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2-LAMP1-mRFP

    Plasmid

    #169004

    Purpose

    Express rat LAMP1-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

    Insert

    rat LAMP1-mRFP
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge