161,813 results

1. pKO403_lacZ_Cm

   Plasmid

   #174725

   Purpose

   Bifidobacterium-E.coli shuttle vector for seamless gene cloning

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB454

   Plasmid

   #74836

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

   Plasmid

   #106171

   Purpose

   cre mediated red to green shift

   Depositor

   Ewa Snaar-Jagalska

   Article

   Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

   Inserts

   dsRED-express2
   eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-M2L

   Plasmid

   #20926

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Inserts

   MYC (MYC Human)
   LIN28 (LIN28A Human)
   

   Use

   Episomal/ebna

   Tags

   Expression

   Mutation

   Promoter

   CMV and IRES

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-HaloTag-KanMX6

   Plasmid

   #87029

   Purpose

   Tag S. pombe genes in C terminal with HaloTag

   Depositor

   Julien Berro

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-GFP

   Plasmid

   #25999

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-GFP (H2BC21 A. victoria, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. RBM10_pLX307

   Plasmid

   #98371

   Purpose

   Lentiviral expression of RBM10

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   RBM10 (RBM10 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   E1Fa

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-myc3-ROC1

   Plasmid

   #20717

   Depositor

   Yue Xiong

   Article

   He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

   Insert

   RING finger protein 1 (ROC1) (RBX1 Human)
   

   Use

   Tags

   3xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-tdPP7-3xmCherry

   Plasmid

   #74926

   Purpose

   Expresses tdPP7-3xmCherry

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   PP7-3xmCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nef-CIAO2-TVA-mCherry

    Plasmid

    #149297

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Start codon removed.

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLH_Scr15

    Plasmid

    #100537

    Purpose

    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    CreC, CreN, and SV40 NLS

    Expression

    Yeast

    Mutation

    N-terminal version of PhyB (AA 1-621)/ Mutation o…

    Promoter

    FBA1 and TDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-rab7 DN

    Plasmid

    #12660

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDawn

    Plasmid

    #43796

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. kiCAP-AAV-MyoAAV4E

    Plasmid

    #224452

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDFNNT insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-SIRT6-H133Y

    Plasmid

    #102327

    Purpose

    Human SIRT6 H133Y for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBF3060

    Plasmid

    #26853

    Purpose

    Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    GAL1

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM19

    Plasmid

    #123655

    Purpose

    Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pIEx

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-FLPo

    Plasmid

    #118027

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-DIO-FLPo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHaloTag-EGFP

    Plasmid

    #86629

    Purpose

    Expresses HaloTag-eGFP tandem (cytosolic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE-puro-GFP-wt-lamin A

    Plasmid

    #17662

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

    Insert

    GFP-lamin A (LMNA Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115216

    Purpose

    Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin2Cas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHCMV_DENV1_PrME

    Plasmid

    #207263

    Purpose

    Mammalian expression of Dengue virus 1 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV1_PrME (POLY Dengue virus 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBb(RSF1010)1k-GFPuv

    Plasmid

    #106395

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMaCTag-05

    Plasmid

    #119984

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO-PTEN-shRNA-1320

    Plasmid

    #25638

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-1320 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-BE2

    Plasmid

    #73020

    Purpose

    Expresses BE2 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO-PTEN-shRNA-3001

    Plasmid

    #25639

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-3001 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti NL

    Plasmid

    #113450

    Purpose

    Lentiviral NanoLuc control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    cmyc

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIRES2_EGFP_KCNQ1

    Plasmid

    #173161

    Purpose

    For mammalian expression of KCNQ1 and creating variants

    Depositor

    Al George

    Article

    Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

    Insert

    KCNQ1 (KCNQ1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115208

    Purpose

    Expresses PinCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PinCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Stargazin-mCherry-FRB

    Plasmid

    #172443

    Purpose

    Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Use

    Tags

    mCherry-HA-FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 HA-YY1

    Plasmid

    #104395

    Purpose

    HA-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1 (YY1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCI-neo Fluc EGFP

    Plasmid

    #90170

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX5X.1 PINK1 WT

    Plasmid

    #13321

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. opto-RhoA-mCherry_pcDNA3.1

    Plasmid

    #164472

    Purpose

    Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

    Insert

    opto-RhoA
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PCDH-WT-ALKBH5-3xFLAG

    Plasmid

    #203736

    Purpose

    Overexpression of ALKBH5-WT

    Depositor

    Jianjun Chen

    Article

    Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

    Insert

    ALKBH5 (ALKBH5 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. YE1-BE4max-NG

    Plasmid

    #138159

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLVX-Cry2olig

    Plasmid

    #208780

    Purpose

    Inducibly expresses light activatable Cry2 domain

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    David Sabatini, Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Nsp3 -mCherry

    Plasmid

    #165131

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp3 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge