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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR

Plasmid

#192446

Purpose

Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP

Plasmid

#37825

Depositor

Edward Boyden

Has Service

AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

Insert

GFP

Use

AAV; Adeno-associated virus

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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v5 ABE-eVLP

Plasmid

#228467

Purpose

Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–ABE8e

Use

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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eeBxb1_IVT

Plasmid

#222342

Purpose

Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Template for in vitro transcription

Tags

Expression

Mutation

Promoter

T7 (inactivated)

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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scramble shRNA

Plasmid

#1864

Purpose

3rd gen lentiviral negative control vector containing scrambled shRNA

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

scramble

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.CAP-B10

Plasmid

#175004

Purpose

non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

Depositor

Viviana Gradinaru

Article

Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

Insert

Synthetic construct isolate AAV.CAP-B10 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

7 amino acid substitution between VP1 452 and VP1…

Promoter

p41

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_COCVmut_G

Plasmid

#207262

Purpose

Mammalian expression of a mutant Cocal virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

COCVmut_G

Use

Tags

Expression

Mammalian

Mutation

K47A/Y209A/R354A; mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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v5 eVLP gag-pro-pol

Plasmid

#228466

Purpose

Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag-pro-pol(Q226P)

Use

Tags

Expression

Mammalian

Mutation

Q226P

Promoter

CMV

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+IMS-HyPer7

Plasmid

#136469

Purpose

Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

SMAC/DIABLO

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHCMV_Bless

Plasmid

#207255

Purpose

Mammalian expression of Baboon Endogenous Retrovirus env protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BaboonEndogenousRetrovirus_Rless_env

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MI-Luciferase-IRES-mCherry

Plasmid

#75020

Purpose

Encodes the expression of Luciferase and mCherry

Depositor

Xiaoping Sun

Article

Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

Inserts

firefly Luciferase
mCherry

Use

Luciferase and Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Tags

Expression

Mutation

Promoter

Cbh

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE N-terminal

Plasmid

#137175

Purpose

AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE N-terminal; U6-protospacer

Use

AAV

Tags

Expression

Mutation

Cas9 D10A

Promoter

Cbh

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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