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pCMV4-p100

Plasmid

#23287

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

NFkB p100 subunit (NFKB2 Human)

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2-N

Plasmid

#41645

Purpose

Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

mNeptune2-Dronpa145N

Expression

Mammalian

Mutation

K145N on Dronpa (allows control of photochromic F…

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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YPET-GGA3

Plasmid

#18841

Depositor

Martin Schwartz

Article

Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Insert

YPET-GGA3 (GGA3 Human)

Tags

His6 and myc

Expression

Mammalian

Mutation

The fluorescent protein YPET was added to the ami…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Has Service

AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Mutation

H134R

Promoter

CAG

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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PKC iota WT

Plasmid

#21252

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC iota (PRKCI Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pMXs-puro Vps34-GFP

Plasmid

#38268

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK1-flag

Plasmid

#15494

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK1 (DKK1 Human)

Tags

flag

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
CyPET-Arf6

Plasmid

#18840

Depositor

Martin Schwartz

Article

Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Insert

CyPET-Arf6 (ARF6 Human)

Expression

Mammalian

Mutation

The sequence for the fluorescent protein CyPET wa…

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only
M-NMn-VP64

Plasmid

#48676

Purpose

Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP c-Abl

Plasmid

#15007

Depositor

Raymond Birge

Insert

c-Abl (Abl1 Mouse)

Tags

IRES EGFP

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-2A-GFP (PX458)

Plasmid

#48138

Purpose

Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pimEJ5GFP

Plasmid

#44026

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ5GFP egfp-based chromosomal break reporter

Expression

Mammalian

Mutation

pCAGGS promoter and eGFP separated by pgkPURO cas…

Promoter

pCAGGS

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 pegRNA2 with trimmed attP

Plasmid

#222347

Purpose

pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA2

Use

CRISPR

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Promoter

1 kb upstream of IFNB1 CDS

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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lentiMPH v2

Plasmid

#89308

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Insert

MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV2/1

Plasmid

#112862

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

James M. Wilson

Insert

Rep2/Cap1

Use

AAV

Promoter

Truncated P5

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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