We narrowed to 26,679 results for: CAT;

1. pcDNA3 Flag TSC2 (S939A)

   Plasmid

   #14132

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 S939A (TSC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S939A.

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. His_Naa80d in pTYB12

   Plasmid

   #188455

   Purpose

   Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   Naa80 (NAA80 Human)
   

   Use

   Tags

   intein-CBD, 6xHis

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hE-cadherin/Δp120

   Plasmid

   #45770

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin/Δp120 (CDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 762-764 converted from EED to AAA to …

   Promoter

   CMV

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

4. AAV8-cPE-C

   Plasmid

   #183539

   Purpose

   Deliver a split compact Prime Editor in vivo

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1A

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET15-His-∆NGlpG

   Plasmid

   #155131

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert codes for GlpG residues 87-276

   Promoter

   T7

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZS*11_4enh

   Plasmid

   #149423

   Purpose

   Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Inserts

   rbcS-E9 enhancer
   35S core enhancer
   Cab-1 enhancer
   AB80 enhancer
   

   Use

   Tags

   T120>A

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-sfGFP-DogTag Loop A

   Plasmid

   #171779

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Val2…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFL-EGFP-3xFlag-DEST

   Plasmid

   #134903

   Purpose

   Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Type

   Empty backbone

   Use

   Tags

   3x-Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedron

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP_mEGFP-GluA1c

   Plasmid

   #114136

   Purpose

   Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   mEGFP tagged GluA1 C-terminus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1 gp78fl

    Plasmid

    #37375

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    P179L and F262L

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HiFi dCas9 VPR

    Plasmid

    #188510

    Purpose

    Expresses FLAG tagged HiFi dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

    Plasmid

    #167902

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MAC-SARS-CoV-2 NSP3

    Plasmid

    #158354

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

    Plasmid

    #199350

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-PLBD2-6xHis

    Plasmid

    #213606

    Purpose

    Expresses PLBD2-6xHis in mammalian cells

    Depositor

    Monther Abu-Remaileh

    Article

    Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

    Insert

    phospholipase B domain containing 2 (PLBD2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sh-RNA hAtg13

    Plasmid

    #31971

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    Atg13 (ATG13 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Dead-Lapronic/pcDNA3.1(-)

    Plasmid

    #140753

    Purpose

    FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio, Negative Control

    Depositor

    Luis Felipe Barros

    Article

    Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

    Insert

    Dead-Lapronic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Histidine 92 to Alanine

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

    Plasmid

    #199351

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-Hoxc6

    Plasmid

    #45553

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc6 (Hoxc6 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pFS16 Pmyo-3 NPP-9 mChBioFLAG

    Plasmid

    #45099

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    npp-9 (npp-9 Nematode)
    unc-119 (unc-119 Nematode)
    

    Use

    Tags

    BLRP, FLAG, and mCherry

    Expression

    Worm

    Mutation

    Promoter

    myo-3 and unc-119

    Available since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQE60 R124K wgMDH

    Plasmid

    #204258

    Purpose

    Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124K

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQE60 R124Q wgMDH

    Plasmid

    #204259

    Purpose

    Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124Q

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQE60 R124A wgMDH

    Plasmid

    #204257

    Purpose

    Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQE60 R130E wgMDH

    Plasmid

    #204267

    Purpose

    Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130E

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA4-Beclin1 1-150

    Plasmid

    #24389

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-150.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

26. AAV8-cPE-N

    Plasmid

    #183538

    Purpose

    Deliver a split compact Prime Editor in vivo

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    N-terminal fragment of SpCas9 nickase (H840A)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AsdCas12a VPR

    Plasmid

    #188512

    Purpose

    Expresses FLAG tagged AsdCas12a VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MBD-IFP2.0C

    Plasmid

    #215748

    Purpose

    Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MBD_R44Q-IFP2.0C

    Plasmid

    #215749

    Purpose

    Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 2xHP1bCD-IFP2.0C

    Plasmid

    #215750

    Purpose

    Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid

    #70658

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3 Flag TSC2 (T1462A)

    Plasmid

    #14130

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 T1462A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T1462A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

33. LbdCas12a VPR

    Plasmid

    #188513

    Purpose

    Expresses FLAG tagged LbdCas12a VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mH2A1.2-CT-GFP

    Plasmid

    #45169

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-NG-PE2-BSD

    Plasmid

    #176933

    Purpose

    Expresses PE2-NG variant in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Insert

    PE2-NG variant
    

    Use

    Lentiviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA/Myc DeltaN TCF4

    Plasmid

    #16513

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta N: lacks aa 1-32

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

37. REEP6-EGFP

    Plasmid

    #210364

    Purpose

    Expresses REEP6 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    REEP6 (REEP6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRelA'

    Plasmid

    #175595

    Purpose

    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Catalytic domain of E.coli relA gene.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Only the catalytic domain of the enzyme is used (…

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FgH1tUTG_mup53_Ex4

    Plasmid

    #85534

    Purpose

    Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge