163,445 results

1. Cyto-V5-APEX2

   Plasmid

   #170572

   Purpose

   Expresses APEX2 in cytosol

   Depositor

   James Olzmann

   Article

   Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

   Insert

   APEX2
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

3. PiggyBac-FLAG-hTET2-IRES-Blast

   Plasmid

   #239819

   Purpose

   PiggyBac vector to express full length human TET2 with 2X N-terminal FLAG tags

   Depositor

   Kasper Rasmussen

   Article

   Knatko et al Blood Adv. 2025 Jun 24:bloodadvances.202

   Insert

   FLAG-tagged human TET2
   

   Use

   Tags

   2x FLAG tag on TET2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Px461-Cas9n-Trp53-sgRNA-alpha

   Plasmid

   #88846

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pU6_HEK3_nsgRNA(PP7)

   Plasmid

   #232444

   Purpose

   nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. JD633

   Plasmid

   #160393

   Purpose

   CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Inserts

   ZmUbi::wheat GRF4-GIF1 chimera
   ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   Binary vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR26 CAG/GFP Asc

   Plasmid

   #74285

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS130

   Plasmid

   #26290

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCE-hSK

    Plasmid

    #41814

    Purpose

    Non-integrating (episomal) expression of human SOX2 and KLF4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    SOX2, KLF4 (SOX2, KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti PGK Blast DEST (w524-1)

    Plasmid

    #19065

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCE-hUL

    Plasmid

    #41855

    Purpose

    Non-integrating (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    L-MYC, LIN28 (MYCL, LIN28A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLIN2-V5-APEX2

    Plasmid

    #170573

    Purpose

    A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    PLIN2 (Plin2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid

    #160729

    Purpose

    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221899

    Purpose

    Expresses alpha tubulin fused to mStayGold

    Depositor

    Janelia Integrative Imaging

    Insert

    mStayGold-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV.CBA.eGFP.2A.P301L-Tau

    Plasmid

    #140425

    Purpose

    AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    changed Proline 301 to Leucine

    Promoter

    CBA

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 HA eIF4GI (1-1599)

    Plasmid

    #45640

    Depositor

    Nahum Sonenberg

    Article

    Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

    Insert

    eIF4GI transcript variant 2 (EIF4G1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pA-His6-Tn5

    Plasmid

    #171871

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Protein A and hyperactive Tn5 transposase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-E-cadherin-RFP

    Plasmid

    #79603

    Purpose

    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    CDH1 promoter region (CDH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge