We narrowed to 15,742 results for: grna

1. pEX-A-U6-MaSgRNA_PuroR

   Plasmid

   #84780

   Purpose

   Expresses major satellite-specific sgRNA.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   major satellite-specific sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-BssgRNA

   Plasmid

   #121957

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   BsCas12b single chimeric gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-U6-AmsgRNA

   Plasmid

   #121956

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AmCas12b single chimeric gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

   Plasmid

   #136659

   Purpose

   Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:TH1477 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript_VEGFA-TS3_SaCas9_sgRNA2

   Plasmid

   #107330

   Purpose

   U6 driven SaCas9 sgRNA expression for VEGFA site 3

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   VEGFA-TS3 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. JARID2-KO-gRNA-1

   Plasmid

   #131328

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

7. JARID2-KO-gRNA-2

   Plasmid

   #131327

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

8. MYC sgRNA5

   Plasmid

   #100557

   Purpose

   Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   MYC sgRNA5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458-sgRNA_Ago1_3

   Plasmid

   #73535

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pVC-Ds-DrU6a:sgRNA-Ds

    Plasmid

    #119069

    Purpose

    To clone sgRNA spacer sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBzCas13b-gRNA-NT

    Plasmid

    #164861

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBzCas13b-gRNA-1

    Plasmid

    #164858

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKDsgRNA-malG

    Plasmid

    #89951

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    malG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EPHB4-gRNA1/2

    Plasmid

    #139974

    Purpose

    gRNA used for knockin EGFP into EPHB4 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    EPHB4 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. EFNB2-gRNA3/4

    Plasmid

    #139973

    Purpose

    gRNA used for knockin tdTomado into EFNB2 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    EFNB2-gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sgRNA EMX1

    Plasmid

    #128121

    Purpose

    Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA EMX1 + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKFSS1_Psyn-sgRNA500

    Plasmid

    #149557

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Psyn and pSyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-sgRNA_Drosha_4

    Plasmid

    #79863

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pMST665_BB3_L 23_gRNAempty_cas9_BbsI

    Plasmid

    #90278

    Purpose

    BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    Cas9
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-sgRNA_Drosha_2

    Plasmid

    #79861

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

21. pX330-sgRNA_Drosha_1

    Plasmid

    #79860

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pKDsgRNA-hisG

    Plasmid

    #89954

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    hisG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC19-U6-AasgRNA3.8.3

    Plasmid

    #121959

    Purpose

    Mammalian expression, Transcription regulation, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, MS2 hairpin inserted
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330-sgRNA_Drosha_3

    Plasmid

    #79862

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

25. pX330-sgRNA_Dicer_1

    Plasmid

    #68807

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHU6_gRNA_Ch10-rev2

    Plasmid

    #81207

    Purpose

    for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 5' of gix site)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MYC sgRNA4

    Plasmid

    #100556

    Purpose

    Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    MYC sgRNA4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEX-A-U6-MiSgRNA_PuroR

    Plasmid

    #84781

    Purpose

    Expresses minor satellite-specific sgRNA.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    minor satellite-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p426_Cas9_gRNA-ARS720a

    Plasmid

    #87392

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS720a sequence CAACAATTGTTACAATAGTA in yeast chromosome 7.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS720a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p426_Cas9_gRNA-ARS1021b

    Plasmid

    #87395

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1021b sequence CCTCTGTGTGGTGGTAATTG in yeast chromosome 10.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1021b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge