We narrowed to 2,903 results for: BFP

1. LV anti-FITC-LaG16-R103A-T2A-BFP

   Plasmid

   #194935

   Purpose

   LV construct encoding R103A mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

   Plasmid

   #120268

   Purpose

   Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

   Depositor

   Edward Callaway

   Article

   Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

   Insert

   EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pU6-mir144 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164791

   Purpose

   Express miR-144 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144 (MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

   Plasmid

   #71213

   Purpose

   dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
   

   Tags

   dSpCas9-BFP-DNMT3A(E752A)

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Ef1a-Con/Foff 2.0-BFP

   Plasmid

   #137130

   Purpose

   Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

   Plasmid

   #102594

   Purpose

   Lentivector to express STING V155M delta 342 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M delta 342

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

   Plasmid

   #129421

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-3Flag
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   3X Flag epitope tag

   Promoter

   none (3X polyA to mitigate episomal expression)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

    Plasmid

    #133328

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #164046

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    mTagBFG-TriTag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

    Plasmid

    #31478

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 K227M T2A BFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    F13Y, S111Y, K227M = Y2 nickase "reo" …

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

    Plasmid

    #128166

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

    Plasmid

    #133333

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All W to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

    Plasmid

    #102595

    Purpose

    Lentivector to express STING V155M delta 354 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 354

    Promoter

    SFFV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CD4-C-BFP HDRT Source (pTR 176)

    Plasmid

    #112020

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-BFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

    Plasmid

    #139230

    Purpose

    mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

    Plasmid

    #87574

    Purpose

    PV1 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CaMKII-mTagBFP2-P2A-DAAO-NES

    Plasmid

    #217566

    Purpose

    Expression of DAAO targeted to the cytoplasm and mTagBFP2, separated by P2A self-cleaving peptide, in mammalian neurons

    Depositor

    Vsevolod Belousov

    Article

    Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

    Insert

    TagBFP2-P2A-DAAO-NES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Oct. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244171

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    IL10Ra signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244174

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    VEGFR1 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244178

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    TNFR1 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244177

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    TNFR2 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

    Plasmid

    #200539

    Purpose

    mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP, NTRv2
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

    Plasmid

    #200540

    Purpose

    mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP, NTRv2
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

    Plasmid

    #200541

    Purpose

    mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP, NTRv2
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMC-ME_2a-BFP-pA ORF-1 without ATG

    Plasmid

    #200534

    Purpose

    mini circle middle entry plasmid for 2a-BFP-pA ORF 1 (without ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMC-ME_2a-BFP-pA ORF-2 without ATG

    Plasmid

    #200535

    Purpose

    mini circle middle entry plasmid for 2a-BFP-pA ORF 2 (without ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMC-ME_2a-BFP-pA ORF-3 without ATG

    Plasmid

    #200536

    Purpose

    mini circle middle entry plasmid for 2a-BFP-pA ORF 3 (without ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    BFP
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTOMM20-Rhobin2-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239022

    Purpose

    Co-overexpression of TOMM20-Rhobin2 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    William DeGrado, Bo Huang

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    TOMM20-Rhobin2 (TOMM20 Synthetic, Human)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTOMM20-Rhobin9-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239023

    Purpose

    Co-overexpression of TOMM20-Rhobin9 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    William DeGrado, Bo Huang

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    TOMM20-Rhobin9 (TOMM20 Synthetic, Human)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRhobin9-SEC61B-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239025

    Purpose

    Co-overexpression of Rhobin9-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    William DeGrado, Bo Huang

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    Rhobin9-SEC61B (SEC61B Synthetic, Human)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid

    #223106

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid

    #223107

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid

    #223108

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid

    #223109

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)

    Plasmid

    #233427

    Purpose

    PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    LIDARsensor(mcherry-BFP)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAS3-rab-3P::AI::lite-1G::SL2::his-24::tagBFP

    Plasmid

    #232610

    Purpose

    Pan-neuronal expression of 'LITE-1' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    LITE-1 (lite-1 Nematode)
    

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP

    Plasmid

    #232608

    Purpose

    Pan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    GUR-3 (gur-3 Nematode)
    

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAGEN-DV-IRES-myr-BFP (JDW 491)

    Plasmid

    #229834

    Purpose

    A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti-hU6-cs1-PuroR-T2A-BFP

    Plasmid

    #230934

    Purpose

    gRNA cloning vector with direct capture sequence 1 in stem loop 2

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid

    #163701

    Purpose

    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Inserts

    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    3x-HA and P2A-tagBFP

    Expression

    Mammalian

    Promoter

    CAG and TRE3G

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pME-myr-mTagBFP2-Flag (JDW 1183)

    Plasmid

    #224492

    Purpose

    Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    myr-mTagBFP2-Flag
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

    Plasmid

    #221082

    Purpose

    Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2
    

    Use

    Synthetic Biology

    Promoter

    CMV, T7

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid

    #219688

    Purpose

    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2BP/p35S (BFP, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid

    #219686

    Purpose

    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

    Plasmid

    #218426

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of both…

    Promoter

    CMV

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)

    Plasmid

    #218425

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of down…

    Promoter

    CMV

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge