We narrowed to 16,478 results for: por

1. pDONR223-MCU-E264A

   Plasmid

   #31730

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Changed E264 to Alanine

   Promoter

   None

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSynapsin- soCoChR-GFP

   Plasmid

   #107708

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Has Service

   AAV9

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVL.SSA TLR (Sce)

   Plasmid

   #45576

   Purpose

   Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with I-Sce I TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185bp, truncated 25 …

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHD-SPARC2-I-LexA::p65

   Plasmid

   #133563

   Purpose

   Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-MCU-E257A

   Plasmid

   #31727

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Changed E257 to Alanine

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-eGFP_mito

   Plasmid

   #221414

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   eGFP_mito
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGK ARF6(Q67L)-CFP

   Plasmid

   #200428

   Purpose

   Mammalian expression of Q67L mutation in ARF6

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   PGK

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-TRKC-V5/HIS

   Plasmid

   #205615

   Purpose

   Expression of human TRKC receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TRKC receptor tyrosine kinase (NTRK3 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWPI-UPP1-3xFLAG

   Plasmid

   #201644

   Purpose

   Over-expression of 3xFLAG-tagged human UPP1

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT2/CAGXSP/PalmReNL

    Plasmid

    #182970

    Purpose

    Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

    Insert

    PalmReNL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-FKBP-GFP-NDP52

    Plasmid

    #135296

    Purpose

    stably express GFP-tagged NDP52 for CID induced mitophagy

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNLF1-C_hCOL4A5-Nluc_R1683X

    Plasmid

    #198124

    Purpose

    Express hCOL4A5_R1683X in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Tags

    Nluc

    Expression

    Mammalian

    Mutation

    R1683X

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV361_MjTyrRS

    Plasmid

    #193250

    Purpose

    Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

    Depositor

    Jessica Seeliger, Peter Schultz

    Article

    Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

    Insert

    MjTyrRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hsp60

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS and P2A

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB.GFP-KIF1A tail 395-1698

    Plasmid

    #134618

    Purpose

    Mammalian expression of mouse KIF1A 395-1698

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 394 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-Rab8a-QL

    Plasmid

    #86076

    Purpose

    A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. APEX2-SOPP3-TM

    Plasmid

    #229077

    Purpose

    Expresses APEX2-SOPP3 in the cell surface

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in the cell surface (APEX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-eGFP_KDEL

    Plasmid

    #221411

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    eGFP_KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiCRISPRv1_SDHB

    Plasmid

    #177981

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHB (SDHB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBa.GFP-KIF1C tail 400-1103

    Plasmid

    #134621

    Purpose

    Mammalian expression of human KIF1C 400-1103

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-RYK-V5/HIS

    Plasmid

    #205610

    Purpose

    Expression of human RYK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RYK receptor tyrosine kinase (RYK Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TRE-UBC-hCD46-T2A-GFP

    Plasmid

    #205457

    Purpose

    lentiviral plasmid for expression of human CD46

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46 (CD46 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFP-Rab8a-TN

    Plasmid

    #86077

    Purpose

    A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SOX17-3'gRNA

    Plasmid

    #210465

    Purpose

    gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    SOX17 (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

    Plasmid

    #90015

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TetO-FUW-PLZF

    Plasmid

    #61543

    Purpose

    Expresses PLZF in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    PLZF (ZBTB16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    I380T and G580D

    Promoter

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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