We narrowed to 21,452 results for: SPR

Showing: 10241 - 10280 of 21452 results

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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AV15_pCAG.Cas9.gRNA.S1

Plasmid

#199259

Purpose

Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

Plasmid

#199262

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle-PR.CC9

Plasmid

#192932

Purpose

pShuttle.CC9 encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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sgCRY1

Plasmid

#176511

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY1-2 (Cry1, Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMb3Cas12a

Plasmid

#174677

Purpose

Expresses 6xHis-MBP tagged Mb3Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMb3Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPb2Cas12a

Plasmid

#174686

Purpose

Expresses 6xHis-MBP tagged Pb2Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPb2Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

Plasmid

#128755

Purpose

Expresses CYRANO gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

CYRANO sgRNA (OIP5-AS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Scara3-IRES-BFP

Plasmid

#117265

Purpose

Retroviral overexpression of Scara3

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Scara3 (Scara3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128340

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna5 (Chrna5 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 CRY2-TSS-guide3

Plasmid

#125491

Purpose

CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 CRY2-TSS-guide2

Plasmid

#125490

Purpose

CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2201

Plasmid

#115449

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2201 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2204

Plasmid

#115450

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2204 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-Q927P4

Plasmid

#103131

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-C7TEQ6

Plasmid

#103092

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-A0LWB3

Plasmid

#103070

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4938

Plasmid

#107527

Purpose

Expresses Fn crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4561

Plasmid

#107525

Purpose

Expresses Lb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-wtSpCas9 (without sgRNA)

Plasmid

#92353

Purpose

Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-wild-type SpCas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE5 (plus strand)

Plasmid

#91841

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0618)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE3 (minus strand)

Plasmid

#91835

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0612)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE3 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE1 (plus strand)

Plasmid

#91837

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0614)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE76

Plasmid

#80578

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAG179 BC072452 3'UTR mut

Plasmid

#12037

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N12 3'UTR mut (FAM107B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pMEDS

Plasmid

#236568

Purpose

Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.

Depositor

Shannon Moonah

Article

Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi

Inserts

CD/UPRT (negative selection)
NPT II (positive selection)

Tags

3x Myc and FLAG

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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sgLATS1/2-2

Plasmid

#229432

Purpose

knockout of LATS1 and LATS2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgLATS1/2-2 (LATS1, LATS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS2700-CMV-Nme2-ABE8e-nt-E932R

Plasmid

#228721

Purpose

Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with Nme2 nCas9 (D16A) E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG eGFP NLS

Plasmid

#170830

Purpose

Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

Plasmid

#191527

Purpose

Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

Depositor

Yannick Doyon

Insert

hGEM(1-110)_mNG_hSLBP(18-108)

Use

CRISPR and Synthetic Biology

Tags

mNeonGreen (mNG) + 3xFLAG_TwinStrep

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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SOX17-NLS-tdTomato-EPG

Plasmid

#210466

Purpose

Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1862

Plasmid

#205975

Purpose

FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1859

Plasmid

#205972

Purpose

FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Csy3/Cas7 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1860

Plasmid

#205973

Purpose

FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6/Csy4 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

Plasmid

#172985

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AV44_pCAG.Cas9-D10A.gRNA.S1

Plasmid

#199258

Purpose

Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9-D10A nickase
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIG-648 CTLA4int1-CTLA4-GFP-SV40

Plasmid

#186123

Purpose

CTLA4 gene knockin

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

WT with GFP sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

Plasmid

#165082

Purpose

gRNA 1 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 21,452 results for: SPR