We narrowed to 29,760 results for: REP

1. pX459V2.0-SpCas9-HF1

   Plasmid

   #108293

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mucin MUC1-5TR-pET15b

   Plasmid

   #174694

   Purpose

   Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

   Depositor

   Jean Gariepy

   Article

   Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

   Insert

   MUC1-TR
   

   Use

   Tags

   hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. DT240

   Plasmid

   #80414

   Purpose

   Expresses 240 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xDBE_RE::Renilla

   Plasmid

   #124535

   Purpose

   Foxo Renilla luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   FOXO Renilla reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETcLIC-GFP

   Plasmid

   #183514

   Purpose

   The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

   Depositor

   Martin Högbom

   Article

   Grave et al Protein Expr Purif. 2022 Oct;198:106132.

   Type

   Empty backbone

   Use

   Tags

   10x His-tag and folding reporter GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SB-SRT-Puro

   Plasmid

   #154888

   Purpose

   Sleeping Beauty self-reporting transposon with puromycin reporter

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   Sleeping beauty SRT
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. DT40

   Plasmid

   #80415

   Purpose

   Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSF1C-A-RFP

   Plasmid

   #172431

   Purpose

   StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

   Depositor

   Simon Moore, Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIY-mETV3-Flag

    Plasmid

    #34579

    Depositor

    Peter Murray

    Article

    El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

    Insert

    Etv3 (Etv3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

11. LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

    Plasmid

    #192406

    Purpose

    To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Turbo GFP
    

    Use

    Synthetic Biology

    Tags

    N7

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF2C-A-RFP

    Plasmid

    #172432

    Purpose

    StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT42_GalL_BE-PAPAPA

    Plasmid

    #145035

    Purpose

    Expresses BE-PAPAPA in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMALp2x-SCO5461

    Plasmid

    #42507

    Depositor

    Koji Okamoto

    Article

    Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

    Insert

    guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
    

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 22, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pJT43_GalL_BE-P(AP)3

    Plasmid

    #145036

    Purpose

    Expresses BE-P(AP)3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-(AP)3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459V2.0-SpCas9-HF4

    Plasmid

    #108295

    Purpose

    pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1‐Afu‐rnhBΔPIP

    Plasmid

    #108696

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C-terminal truncation (7aa) removing PIP box

    Promoter

    tac

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1‐Afu‐rnhB D101N

    Plasmid

    #108695

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D101N catalytic site mutation

    Promoter

    tac

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Luc-Nanog-3'UTR

    Plasmid

    #63893

    Purpose

    Luciferase reporter of mouse wildtype Nanog 3'UTR

    Depositor

    Lin He

    Article

    Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ_NAND2_L17_S19_S11

    Plasmid

    #132730

    Purpose

    T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_NAND2_L17_S19_S11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIK137

    Plasmid

    #65632

    Purpose

    Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJT23_GalL_BE-PAPAP

    Plasmid

    #143486

    Purpose

    Expresses BE-PAPAP in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMIY-mETV3

    Plasmid

    #34578

    Depositor

    Peter Murray

    Article

    El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

    Insert

    Etv3 (Etv3 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1-mETV3

    Plasmid

    #33374

    Depositor

    Peter Murray

    Article

    El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

    Insert

    Etv3 (Etv3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pET24a_UDP_Xase_E113

    Plasmid

    #73159

    Purpose

    Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

    Depositor

    Sandra Gabelli

    Article

    Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

    Insert

    pET24a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    change glutamate 113 to alanine

    Promoter

    Available Since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pMCh-139

    Plasmid

    #92287

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

    Insert

    dGW182 NED (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 NED: aa 205-490

    Promoter

    Ac5

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Luc-Nanog-3'UTRmut

    Plasmid

    #63894

    Purpose

    Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

    Depositor

    Lin He

    Article

    Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3.1-mETV3-HA

    Plasmid

    #33375

    Depositor

    Peter Murray

    Article

    El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

    Insert

    Etv3 (Etv3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pFP121

    Plasmid

    #21670

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pHAGE2-TetO-mCherry

    Plasmid

    #216174

    Purpose

    Generate lentiviruses to act as a positive transduction control for pHAGE2-TetO vectors in pluripotency reprogramming

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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