166,027 results

1. pInducer10b-EGFP-KRAS-WT

   Plasmid

   #164935

   Purpose

   Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

   Inserts

   Enhanced green fluorescent protein
   KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
   

   Use

   Lentiviral

   Promoter

   Ubc Promoter and Ubc promoter

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-hCCND1-HA

   Plasmid

   #174154

   Purpose

   Lentiviral vector expressing HA-tagged human cyclin D1

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   hemagglutinin (HA)

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

   Plasmid

   #216247

   Purpose

   Express OsTIR1.F74A mutant with mCherry tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   OsTIR1.F74A
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

   Plasmid

   #194715

   Purpose

   Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE-BI-osTIR1

   Plasmid

   #207840

   Purpose

   Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   osTIR
   mCherry
   mAID_-VHH-GFP4
   

   Tags

   3X HA-Tag and weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-TetO-Nr5a1

   Plasmid

   #41081

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

   Insert

   Nr5a1 (Nr5a1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TetO + minimal CMV

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS40H

   Plasmid

   #35479

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB_NPY1.0

   Plasmid

   #208676

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBQ10-GW

    Plasmid

    #209370

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by UBQ10 promoter

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB405-TIC21-sGFP

    Plasmid

    #213865

    Purpose

    Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

    Insert

    chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
    

    Expression

    Bacterial and Plant

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGKB

    Plasmid

    #85077

    Purpose

    expression in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

    Plasmid

    #231973

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti UBC hNLRP3-mNG

    Plasmid

    #218642

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-GRAB_VIP1.0

    Plasmid

    #208681

    Purpose

    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTn7-SCOUT11

    Plasmid

    #200991

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. hHSF1 RNAi resistant

    Plasmid

    #118347

    Purpose

    This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    RNAi resistance mutations (does not change amino …

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MT-AvrXa10-FT

    Plasmid

    #234373

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

    Depositor

    Michael Smanski

    Article

    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

    Inserts

    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    

    Use

    Synthetic Biology

    Tags

    GB1, GFP, and GP41

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. opto-Itsn1

    Plasmid

    #174509

    Purpose

    Expresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Itsn1-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. (SUMO)10-(SIM)5

    Plasmid

    #126866

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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