We narrowed to 2,903 results for: BFP

1. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP

   Plasmid

   #212106

   Purpose

   Expression of GABARAP split tandem protein with mTagBFP2

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   G116A (lipidation deficiency) of upstream GABARAP

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP

   Plasmid

   #212107

   Purpose

   Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   5X: K2A, K13A, R15A, K20A, K23A mutations of upst…

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

   Plasmid

   #212109

   Purpose

   Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Human, Entacmaea quadricolor)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP

   Plasmid

   #216476

   Purpose

   lentiviral dual expression of dsRed and tagBFP

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   dsRed
   tagBFP
   

   Use

   Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(12))-PGKpuroBFP-W

   Plasmid

   #208569

   Purpose

   Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   RUNX1(13), RUNX3(12) (RUNX1, RUNX3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-W

   Plasmid

   #208570

   Purpose

   Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   RUNX1(13), RUNX3(13) (RUNX1, RUNX3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(21))-PGKpuroBFP-W

   Plasmid

   #200507

   Purpose

   Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   ARID1A(44), ARID1B(21) (ARID1A, ARID1B Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

   Plasmid

   #200506

   Purpose

   Lentiviral vector expressing gRNA targeting human ARID1A and AAVS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   ARID1A(44), AAVS1 (ARID1A, AAVS1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-W

   Plasmid

   #200508

   Purpose

   Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   ARID1A(44), ARID1B(23) (ARID1A, ARID1B Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #200502

    Purpose

    Lentiviral vector expressing gRNA targeting human AAVS1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    AAVS1 (AAVS1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

    Plasmid

    #200503

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

    Plasmid

    #200505

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP

    Plasmid

    #202826

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

    Plasmid

    #202825

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #199450

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #199453

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

    Plasmid

    #195501

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

    Plasmid

    #178871

    Purpose

    Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

    Plasmid

    #167921

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

    Plasmid

    #178870

    Purpose

    Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

    Plasmid

    #139237

    Purpose

    mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

    Plasmid

    #139234

    Purpose

    mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

    Plasmid

    #139233

    Purpose

    mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

    Plasmid

    #139232

    Purpose

    mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

    Plasmid

    #139231

    Purpose

    mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

    Plasmid

    #156404

    Purpose

    Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mTagBFP2

    Mutation

    mutant KRAS4A-G12D

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

    Plasmid

    #128764

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #3 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

    Plasmid

    #128763

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #2 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

    Plasmid

    #128762

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #1 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

    Plasmid

    #128760

    Purpose

    Expresses PARN gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PARN sgRNA #2 (PARN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

    Plasmid

    #128758

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #3 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

    Plasmid

    #128756

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

    Plasmid

    #128757

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

    Plasmid

    #117137

    Purpose

    Expression vector encoding BFP-BSD-Nkx2-5

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

    Plasmid

    #219548

    Purpose

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
    

    Expression

    Mammalian

    Mutation

    The (SAGG)x4 linker between GAL4(1-65) and the PC…

    Promoter

    CMVd1

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

    Plasmid

    #170273

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
    

    Expression

    Mammalian

    Mutation

    See Comments

    Promoter

    CMVd1

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid

    #176027

    Purpose

    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    rtTA3
    Luc2
    mtagBFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158653

    Purpose

    making transgenics expressing a UAS driven mCherry and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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40. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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41. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

    Plasmid

    #188767

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200402

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]

    Plasmid

    #133325

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of CTD in full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221076

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221077

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221079

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]

    Plasmid

    #133324

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of RRM in full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge