We narrowed to 8,809 results for: CAG

1. pCAG-BirA*-dCas9-eGFP

   Plasmid

   #84779

   Purpose

   Expresses BirA*-dCas9-eGFP in mammalian cells.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   BirA*-dCas9-eGFP
   

   Use

   CRISPR

   Tags

   cMyc

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB CAG-mTagBFP2-T2A-iCreERT2

   Plasmid

   #177308

   Purpose

   CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hGPR56-IRES-GFP

   Plasmid

   #52297

   Purpose

   Expresses human GPR56 and GFP in mammalian cells.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   Human GPR56 coding sequence (ADGRG1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-Nst-CAG-DEST

   Plasmid

   #80489

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209653

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG dCas9-KRAB-2A-EGFP

   Plasmid

   #92396

   Purpose

   CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9_KRAB_2A_EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-MLC2(CA)-IRES-GFP

   Plasmid

   #133928

   Purpose

   Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EGFP and MLC2(CA) (MYL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MLC2 T18D, S19D

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS SARS-CoV-2 BA.4/5 Spike

   Plasmid

   #186031

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

   Insert

   SARS-CoV-2 BA.4/5 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pAAV-CAG-DIO-EYFP

   Plasmid

   #104052

   Purpose

   An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.V1

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZ M2rtTA_CAGG TetON-Sox10 with GFP

    Plasmid

    #115241

    Purpose

    RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Inserts

    SOX10 (SOX10 Human)
    eGFP
    

    Use

    Tags

    GFP (separate polypeptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY0750 pCAG-NLS-BceINT

    Plasmid

    #179112

    Purpose

    BceINT expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-N-TEV-Twin-Strep

    Plasmid

    #202526

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-mNaChBac-T2A-tdTomato

    Plasmid

    #60650

    Purpose

    Expresses mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    mNaChBac-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV2-CAGIG-WGA-mCherry

    Plasmid

    #224119

    Purpose

    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.

    Depositor

    Pantelis Tsoulfas

    Insert

    WGA-mCherry
    

    Use

    AAV

    Tags

    WGA and mCherry

    Expression

    Mammalian

    Mutation

    All mutations related to the WGA in this construc…

    Promoter

    CAGIG

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-C-TEV-Twin-Strep

    Plasmid

    #202527

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-U6-dgRNA-CAG-MPH

    Plasmid

    #106259

    Purpose

    Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    MS2-P65-HSF1 (HSF1 Human, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-NIR-GECO2G

    Plasmid

    #159605

    Purpose

    AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2G

    Depositor

    Robert Campbell

    Article

    Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    NIR-GECO2G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG R2pH-LAMP1-3xFLAG

    Plasmid

    #157940

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Chick beta actin pomoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-fDIO-mNeonGreen

    Plasmid

    #99133

    Purpose

    An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    fDIO-mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209657

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95A.A119L

    Promoter

    CAG

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-CAG-jGCaMP8m-WPRE

    Plasmid

    #179255

    Purpose

    AAV-mediated expression of GCaMP8m under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV CAG-DO-TC66T-mCherry

    Plasmid

    #175178

    Purpose

    Cre-off TVA-mCherry fusion protein with 66T mutation

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    TVA
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    E66T

    Promoter

    CAG

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pR26 CAG/BFP Dest

    Plasmid

    #74282

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLR5-CAG-TetR_W43F-3xmNG

    Plasmid

    #174880

    Purpose

    PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    TetR(W43F)-3xmNeonGreen
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-Oct3/4-IRES-Neo

    Plasmid

    #13461

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P31S

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG_GST-TEV-EGFP-ATG13

    Plasmid

    #223797

    Purpose

    Expression of recombinant protein for purification

    Depositor

    James Hurley, Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST-TEV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS SARS-CoV-2 BA.1 Spike

    Plasmid

    #185452

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

    Depositor

    Marceline Côté

    Article

    Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

    Insert

    SARS-CoV-2 Spike BA.1 (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: A67V, Δ69-70, T…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pCAGGS SARS-CoV-2 BA.2 Spike

    Plasmid

    #185604

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

    Insert

    SARS-CoV-2 BA.2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    July 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pCAG-MCS-TurboID-3xFLAG

    Plasmid

    #186814

    Purpose

    CAG promoter-driven expression of BioID2-fused protein (C terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Use

    Tags

    TurboID-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-LplA(AILR,36)-NRX3b

    Plasmid

    #43919

    Depositor

    Alice Ting

    Article

    Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

    Insert

    AILR-LplA36-NRX3b (lplA Human, Synthetic)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-CAG-Chronos-GFP

    Plasmid

    #99232

    Purpose

    AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pR26-CAG-H2B-miRFP703

    Plasmid

    #112853

    Purpose

    Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    H2B-miRFP703
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT/Caggs-NRAS G12V/D38A

    Plasmid

    #221073

    Purpose

    Expresses NRAS G12V/D38A in mammalian cells

    Depositor

    Hidenori Ichijo

    Article

    Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

    Insert

    NRAS (NRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V/D38A

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-SpCas9-GFP-U6-gRNA

    Plasmid

    #79144

    Purpose

    All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

    Depositor

    Jizhong Zou

    Article

    Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Use

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAGGS/XBB.1.16-RBD

    Plasmid

    #213070

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/XBB.1.16-RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-TET3G-T2A-Puro

    Plasmid

    #158067

    Purpose

    Encodes the Tet3G transactivator and puromycin resistance.

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    pCAG-TET3G-T2A-Puro
    

    Use

    AAV

    Tags

    puromycin reistance gene

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid

    #191097

    Purpose

    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-vsfGFP-0
    

    Use

    AAV

    Tags

    Green fluorescent protein bound to enhancer nanob…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pPB CAG SMARCA4-Venus

    Plasmid

    #153950

    Purpose

    piggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion protein

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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42. pAAV-CAG-FLEX-SomaGCaMP7

    Plasmid

    #182942

    Purpose

    Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    Soma-GCaMP7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pCAG-GluA1-scFV-Halo

    Plasmid

    #230065

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. pLKO.1-puro U6 sgRNA CAG

    Plasmid

    #50927

    Purpose

    U6 driven sgRNA negative control

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    negative control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E174R, R951K, R955A

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. AAV-CAG-DIO-APEX2NES

    Plasmid

    #79907

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    APEX2NES
    

    Use

    AAV and Cre/Lox

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCAG-i53-bpA-EF1BFP

    Plasmid

    #111145

    Purpose

    Expression of i53 and a BFP reporter in mammalian cells

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Inserts

    i53 (mutL Synthetic)
    BFP (BFP Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human Ubiquitin, contains 8 point mutations

    Promoter

    CAG promoter and EF1

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. AAVS1-SA-2A-NEO-CAG-RTTA3

    Plasmid

    #60431

    Purpose

    neomycin resistance; constitutive promoter driving reverse tet activator

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    rtTA3
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV-CAG-ArchT-GFP

    Plasmid

    #29777

    Purpose

    AAV-mediated expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-CAG-HcKCR2-EYFP

    Plasmid

    #182022

    Purpose

    Express light-gated potassium channel HcKCR2-EYFP in mammalian cells

    Depositor

    Mingshan Xue

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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