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Showing: 1251 - 1300 of 2950 results

  1. pF(UG) hSyn NS34a-msGFP-'Q-mito

    Plasmid
    #158767
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158767-plasmid-map-sequence-id-313145

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  2. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid
    #158768
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158768-plasmid-map-sequence-id-313146

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  9. UAS:mCherry

    Plasmid
    #170994
    Purpose
    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    UAS:mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic 14x UAS:Gal4
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170994-plasmid-map-sequence-id-335660

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  17. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-457342

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  20. pYPQ202

    Plasmid
    #86198
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis ubiquitin 10 (AtUbi10)
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86198-plasmid-map-sequence-id-174281

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  26. pGal4x5-E4T-G210

    Plasmid
    #171077
    Purpose
    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Insert
    Gal4-E4-Gless-cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171077-plasmid-map-sequence-id-335554

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  29. G-CaMP3

    Plasmid
    #22692
    Purpose
    a single-wavelength GCaMP2-based genetically encoded calcium indicator
    Depositor
    Loren Looger
    Article
    Tian et al Nat Methods. 2009 Dec;6(12):875-81.
    Insert
    G-CaMP3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N363D mutation (increased fluorescence)
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    22692-plasmid-map-sequence-id-241043

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  47. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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Showing: 1251 - 1300 of 2950 results