We narrowed to 75,658 results for: Rest

1. pTYB-pLAC-F112S

   Plasmid

   #48727

   Purpose

   Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

   Depositor

   Gordon Laurie

   Article

   Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

   Insert

   Lacritin (LACRT Human)
   

   Use

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   Phenylalanine 12 to Serine

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pTYB1-pLAC-I98S

   Plasmid

   #48723

   Purpose

   Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

   Depositor

   Gordon Laurie

   Article

   Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

   Insert

   Lacritin (LACRT Human)
   

   Use

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   Isoleucine 98 to Serine

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pTYB1-pLAC-I73S

   Plasmid

   #48722

   Purpose

   Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

   Depositor

   Gordon Laurie

   Article

   Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

   Insert

   Lacritin (LACRT Human)
   

   Use

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   Isoleucine 73 to Serine

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pTYB1-pLAC-V69S

   Plasmid

   #48721

   Purpose

   Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

   Depositor

   Gordon Laurie

   Article

   Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

   Insert

   Lacritin (LACRT Human)
   

   Use

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   Valine 69 to Serine

   Promoter

   T7

   Available since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pWPI-N3ICD

   Plasmid

   #185524

   Purpose

   Constitutive overexpression of human NOTCH3 intracellular domain

   Depositor

   Eric Shusta, Sean Palecek

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH3 intracellular domain (NOTCH3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH_CMV_TFAM_mKate2

   Plasmid

   #167331

   Purpose

   Expresses mKate2 fused to full length TFAM in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-TetOne-Puro-EcSTH-FLAG

   Plasmid

   #218628

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   EcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-TetOne-Puro-mitoEcSTH-FLAG

   Plasmid

   #218629

   Purpose

   This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoEcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDH_blast_MCS_Nard_GFP_LAMIN

   Plasmid

   #167340

   Purpose

   Expresses GFP fused to lamin A (LA) in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO-RB1-shRNA63

    Plasmid

    #25641

    Purpose

    3rd generation lentiviral vector

    Depositor

    Todd Waldman

    Article

    Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

    Insert

    RB1-shRNA 63 (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PKT2/CLP-AKT

    Plasmid

    #20281

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    AKT (AKT1 Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO-RB1-shRNA19

    Plasmid

    #25640

    Purpose

    3rd generation lentiviral vector

    Depositor

    Todd Waldman

    Article

    Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

    Insert

    RB1-shRNA 19 (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Eric Shusta, Sean Palecek

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-p53 2KR

    Plasmid

    #78799

    Purpose

    To overexpress p53 2KR in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K291R, K292R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

15. PT3.5/CMV-EGFRvIII

    Plasmid

    #20280

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    EGFRvII (EGFR Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    See depositor comments below.

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEMS1932

    Plasmid

    #79781

    Purpose

    High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple259
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple259 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEMS1937

    Plasmid

    #79782

    Purpose

    High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple260
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple260 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. β₂AR-mCFP

    Plasmid

    #38260

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    β₂AR (ADRB2 Human)
    

    Use

    Tags

    Flag, mCFP, and signal peptide sequence (MKTIIALS…

    Expression

    Mammalian

    Mutation

    diglycine linker (replacing terminator codon)

    Promoter

    CMV

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCB19

    Plasmid

    #194757

    Purpose

    EGFP-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    EGFP-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCB20

    Plasmid

    #194758

    Purpose

    ALFA-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    ALFA-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSEMS-CV SUy-Halo7Tag

    Plasmid

    #108933

    Purpose

    Selflabeling tag at ATP Synthase

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    ATP synthase F1 subunit gamma (ATP5F1C Human)
    

    Use

    Tags

    Halo7 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCIneoMyc UNC119B

    Plasmid

    #128333

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119B

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119B (UNC119B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCIG-VP16–RAR-alpha

    Plasmid

    #80434

    Purpose

    Expression of constitutively active human RAR-alpha in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Retinoic acid receptor alpha (RARA Human)
    

    Use

    Tags

    EGFP and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

25. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. alpha-0 CFP in pcDNA3.1

    Plasmid

    #36396

    Depositor

    Narasimhan Gautam

    Article

    Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

    Insert

    G protein alpha 0 (Gnao1 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted into the KpnI-SpeI sites engineered …

    Promoter

    cmv

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

27. MIGR1-cyclin E-AA

    Plasmid

    #47498

    Purpose

    MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons

    Depositor

    Alex Minella

    Article

    Xu et al Oncogene. 2013 Jul 22. doi: 10.1038/onc.

    Insert

    cyclin E1 (CCNE1 Human)
    

    Use

    Retroviral; Mscv-ires-gfp

    Tags

    Expression

    Mammalian

    Mutation

    threonines 62 and 380 to alanine (T62A T380A)

    Promoter

    LTR

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pLVX-Phi3-Zsgreen-PGK-mCherry

    Plasmid

    #153320

    Purpose

    Expresses zsGreen and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    zsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX459-HypaCas9-ANLN_sgRNA

    Plasmid

    #183874

    Purpose

    pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX459-HypaCas9-ECT2_sgRNA

    Plasmid

    #183873

    Purpose

    pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ECT2 sgRNA spacer (ECT2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOPINK-OTUD4 (OTU, aa 22-196)

    Plasmid

    #61413

    Purpose

    Expresses human OTUD4 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-21 and aa 197-1114.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

    Plasmid

    #61582

    Purpose

    Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne2 (OTUD7A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Isoform 2. Deleted aa 463-933.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Beta2-AR-TYY

    Plasmid

    #32694

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2006 Jan 13;281(2):1261-73.

    Insert

    β2AR (ADRB2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T68F, Y132G Y219A

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

34. 1037 pGL3 FasL promoter

    Plasmid

    #9028

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA Myc-mMEKK2

    Plasmid

    #44158

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    Mekk2 (Map3k2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pLenti-sgRELA

    Plasmid

    #83944

    Purpose

    Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgRELA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 1129 pcDNA3 GFP FKHR H215R AAA

    Plasmid

    #9034

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3.1 Myc-huMEKK3 (K391A)

    Plasmid

    #44157

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    MEKK3 (K391A) (MAP3K3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K391A, kinase-dead mutant

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

39. E12-cTAP

    Plasmid

    #20916

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E12 cTAP (Tcf3 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    TAP tag is fused to the 3' end of the E12 ge…

    Promoter

    Available since

    May 15, 2009

    Availability

    Academic Institutions and Nonprofits only

40. pBV-Luc/Del-6

    Plasmid

    #14969

    Depositor

    Joan Massague

    Article

    Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

    Insert

    c-myc promoter (-109/+334) (MYC Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -109/+334 (relative to the P2 transcription initi…

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

41. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. 20XUAS IVS Jaws mCherry_tr

    Plasmid

    #111549

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. HOXB7 (human) pCMV

    Plasmid

    #8539

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

45. Th-iCre

    Plasmid

    #159459

    Purpose

    Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    P2A-iCre
    

    Use

    Cre/Lox

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous TH

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 10XUAS IVS Jaws mVenus_tr

    Plasmid

    #111553

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mVenus_tr
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. HOXB4 (human) FLAG MSCV

    Plasmid

    #8528

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Missing N-terminal 14 amino acids; improved Ribos…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

49. pEMS2044

    Plasmid

    #79652

    Purpose

    ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple255-EmGFP WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    PAX6 HS234Z Enhancer

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. 13XLexAop2 IVS Jaws mCherry_tr

    Plasmid

    #111551

    Purpose

    Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge