We narrowed to 987 results for: Streptococcus

1. gRNA NIA TLS1

   Plasmid

   #196980

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS1 tags
   

   Expression

   Plant

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AV15_pCAG.Cas9.gRNA.S1

   Plasmid

   #199259

   Purpose

   Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRH2502

   Plasmid

   #84379

   Purpose

   Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   dcas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Aspartate at position 10 changed to Alanine, Hist…

   Promoter

   uv15tetO

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMT1-24X-NOG(AtUBI10)

   Plasmid

   #202016

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Inserts

   dCas9-24XGP41
   NbGP41P-sfGFP-VP64-GB1
   

   Tags

   GB1 and sfGFP

   Expression

   Plant

   Promoter

   AtUBI10

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNHEJ-Cas9-recX

   Plasmid

   #158715

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   cas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   TetO

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

   Plasmid

   #218513

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of the Plac_mrfp cassette

   Promoter

   PgyrA_ermBL-ermB'

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Flag-hGSDMD

   Plasmid

   #218938

   Purpose

   Expression of N-terminal FLAG tagged human gasdermin D

   Depositor

   Christopher LaRock

   Article

   LaRock et al Nature. 2022 May;605(7910):527-531. doi:

   Insert

   human GSDMD (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEC3006 (pSpy1C-PZn_ffluc)

   Plasmid

   #218515

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PZn (PczcD)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pspCas9-HF-tetra-com-vector

    Plasmid

    #132555

    Purpose

    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Expression

    Mammalian

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS315e_pGal-nCas9(D10A)-PmCDA1

    Plasmid

    #79617

    Purpose

    Expresses nCas9(D10A)-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJIT163-2NLSCas9

    Plasmid

    #53064

    Purpose

    Expression of rice codon-optimized Cas9 in plant cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    Cas9
    

    Use

    Plant expression

    Mutation

    Rice codon optimized

    Promoter

    2x35S

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS315e_pGal-dCas9-PmCDA1

    Plasmid

    #79615

    Purpose

    Expresses dCas9-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRB14

    Plasmid

    #52522

    Purpose

    expresses a myc-tagged version of hCas9 in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    S. pyogenes cas9 with humanized codon bias
    

    Tags

    myc

    Expression

    Insect

    Promoter

    tubulin

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJT130_GalL_nCDA1-NGBE3

    Plasmid

    #145094

    Purpose

    Expressing base editor nCDA1-NGBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-NGBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Expression

    Mammalian

    Promoter

    CMV enhancer-rat EF1 alpha

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT1-24X-NOG(CmYLCV)

    Plasmid

    #202014

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Promoter

    CmYLCV

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HiFi dCas9 KRAB

    Plasmid

    #188500

    Purpose

    Expresses FLAG tagged HiFi dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEasyG1_hph

    Plasmid

    #184912

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge