We narrowed to 3,217 results for: Streptococcus

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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Publication

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEPQD1CB0202

Plasmid

#185626

Purpose

MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:fflucRT

Plasmid

#88901

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Publication

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + Red-shifted firefly luciferase

Expression

Bacterial

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPEPY

Plasmid

#122633

Purpose

BglFusion compatible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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PX544 SpCas9(del1098-1368)

Plasmid

#58889

Purpose

Truncated form of SpCas9 without C' domain

Depositor

Feng Zhang

Publication

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 1098-1368

Available since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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PX543 SpCas9(del175-307)

Plasmid

#58888

Purpose

Truncated form of SpCas9 without REC2 domain

Depositor

Feng Zhang

Publication

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 175-307

Available since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pPEPX-Plac

Plasmid

#122632

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Expression

Mammalian

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPEPY-Plac

Plasmid

#122634

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPEPX

Plasmid

#122631

Purpose

BglFusion compatible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEC3135 (pERASE)

Plasmid

#220306

Purpose

Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

Depositor

Emmanuelle Charpentier

Publication

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Synthetic Biology

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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HA-L-SA-2A-NeoR-CAG-M2rtTA-Cas9-TRE-HA-R

Plasmid

#258168

Purpose

DNA template for insertion of doxycycline inducible Cas9 and constitutively active M2rtTA expression cassettes into human AAVS1 site

Depositor

Danwei Huangfu

Publication

Zhang et al Dev Cell. 2026 May 19:S1534-5807(26)0015

Insert

SpCas9

Tags

Flag tagged

Expression

Mammalian

Promoter

TRE

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Publication

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGBSedit Sham

Plasmid

#223200

Purpose

Group B Streptococcus Efficient Genome Editing with Modular crRNA

Depositor

Thomas Hooven

Publication

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

CRISPR

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0001

Plasmid

#185625

Purpose

MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pASR103

Plasmid

#122637

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Publication

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTG131

Plasmid

#198640

Purpose

Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

Depositor

David Bikard

Publication

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

Cas9

Expression

Bacterial

Promoter

pPhlF

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKB01_tagRFP

Plasmid

#69103

Purpose

Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Publication

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

tagRFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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