We narrowed to 987 results for: Streptococcus
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Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
ExpressionPlantPromoterpU6-26, pU6-29Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV15_pCAG.Cas9.gRNA.S1
Plasmid#199259PurposeExpression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNADepositorInsertsSpCas9 nuclease
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCAG promoter and RNA polymerase III promoter for …Available SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH2502
Plasmid#84379PurposeExpression of dcas9 D10A H840A from a TetR-regulated uvtetO promoterDepositorInsertdcas9
UseCRISPRExpressionBacterialMutationAspartate at position 10 changed to Alanine, Hist…Promoteruv15tetOAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR_Cas9_sp1
Plasmid#154851PurposeExpresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genomeDepositorInsertsSpcas9 gene
sgRNA scaffold
UseCRISPRTags6xHisMutationCodon harmonized variant for expression in Rhodob…Available SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(AtUBI10)
Plasmid#202016PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
TagsGB1 and sfGFPExpressionPlantPromoterAtUBI10Available SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRExpressionBacterialPromoterTetOAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-hGSDMD
Plasmid#218938PurposeExpression of N-terminal FLAG tagged human gasdermin DDepositorAvailable SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-HF-tetra-com-vector
Plasmid#132555PurposeVector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.DepositorInsertsHigh fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop
ExpressionMammalianAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKMD106e - intein-Cas9(S219)
Plasmid#64190PurposeExpresses intein-Cas9(S219) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-nCas9(D10A)-PmCDA1
Plasmid#79617PurposeExpresses nCas9(D10A)-PmCDA1 in yeast cellsDepositorInsertSpCas9
TagsPmCDA1ExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionMutationRice codon optimizedPromoter2x35SAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-PmCDA1
Plasmid#79615PurposeExpresses dCas9-PmCDA1 in yeast cellsDepositorInsertSpCas9
TagsPmCDA1ExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRB14
Plasmid#52522Purposeexpresses a myc-tagged version of hCas9 in DrosophilaDepositorInsertS. pyogenes cas9 with humanized codon bias
TagsmycExpressionInsectPromotertubulinAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRai
Plasmid#189938PurposeExpresses CRISPRai cassette under EF1a promoterDepositorInsertCRISPRai cassette
ExpressionMammalianPromoterCMV enhancer-rat EF1 alphaAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(CmYLCV)
Plasmid#202014PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
TagsGB1 and sfGFPExpressionPlantPromoterCmYLCVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiFi dCas9 KRAB
Plasmid#188500PurposeExpresses FLAG tagged HiFi dCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A/R691A/H840APromoterEF1aAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only