We narrowed to 171,429 results for: addgene
-
Plasmid#184445PurposeSingle EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and Ef1AAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only
-
FH1-MGT#2-mCherry_PGK-H2B-CFP
Plasmid#164102PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#2-mCherry
UseLentiviral and Synthetic BiologyAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRECBRR1-Kva1
Plasmid#240703Purposeinverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, PaRecT and PaSSB
ExpressionBacterialMutationInverted BRR1 origin of replicationAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pREC1010-Kva1
Plasmid#240684PurposeRSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoterDepositorInsertKva1 RT,Kva1 ncRNA, Beta and EcSSB
ExpressionBacterialMutationWTAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
[#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient
Plasmid#233506PurposeLentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cellsDepositorInsertDsRed loxp eGFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA H1047R
Plasmid#12524PurposeRetroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationH1047R. Tumor-derived allele.Available SinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNoROS-CAT
Plasmid#170152PurposeMammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidationDepositorInsertsUseGenome integration via sleeping beauty transposon…TagsFLAG tag and Nucleolar Localization Sequence (NoL…ExpressionMammalianPromoterRPBSA and TRE promoterAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide
Plasmid#117986PurposepLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.DepositorInsertsgRNA backbone (F+E)
UseLentiviralAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
hHS1-M7A,H102A
Plasmid#159171PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1-M7A,H102A
ExpressionMammalianMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterCMVAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro CCND2-T2A-luciferase-P2A-eGFP
Plasmid#179843PurposeTunable and temporal expression control of CCND2, luciferase and nuclear eGFPDepositorInsertCCND2 (CCND2 Human)
UseLuciferase and Synthetic BiologyTagseGFP and luciferaseExpressionMammalianPromoterTRE3GAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh and U6Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2_ISCro4_Insertion_Reporter
Plasmid#251500PurposeReporter plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and pDonor_ISCro4_(WT)_Donor_ECFP plasmidDepositorInsertISCro4 insertion reporter
ExpressionMammalianMutationNAAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tol2_ISCro4_Deletion_Reporter
Plasmid#251498PurposeReporter plasmid for monitoring deletion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL componentsDepositorInsertISCro4 deletion reporter
ExpressionMammalianMutationNAAvailable SinceFeb. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tol2_ISCro4_Inversion_Reporter
Plasmid#251499PurposeReporter plasmid for monitoring inversion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL componentsDepositorInsertISCro4 inversion reporter
ExpressionMammalianMutationNAAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-AAVR-FLAG
Plasmid#166716PurposeVector expressing AAVR (KIAA0319L) with a C-terminal Flag tagDepositorAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_3xFLAG-ATP6V0A3-mScarlet
Plasmid#228867PurposeStable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarletDepositorAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_3XHA-ATP6V1B2-mNeonGreen
Plasmid#228865PurposeStable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreenDepositorAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305-C-dTAG
Plasmid#91798PurposeLentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG systemDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianPromoterhPGKAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG
Plasmid#81021PurposeCancer-associated ASXL1 truncation mutant in retroviral vectorDepositorInsertASXL1 (ASXL1 Human)
Tags3X FLAGExpressionMammalianMutationp.G646 Wfs *12 truncationPromoter5’LTRAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-SpyCatcher003-mNeonGreen
Plasmid#234543Purposeto express a green fluorescent protein tagged with SpyCatcher in the liverDepositorInsertIgKL-SpyCatcher003-mNeonGreen
UseAAVMutationNonePromoterP3Available SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS hygro
Plasmid#158634PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS constructDepositorAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
FpG5
Plasmid#69443Purposelentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAsDepositorInsertsminimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
UseLentiviralExpressionMammalianAvailable SinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
NPRL2_pLX307
Plasmid#98364PurposeLentiviral expression of NPRL2DepositorAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianPromoterSFFVAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pCAGGS_hTRPV1-p2A-tdTomato
Plasmid#238923PurposeExpression of the human TRPV1 channel with tdTomato as a reporter in mammalian cellsDepositorInserthTRPV1-p2A-tdTomato
ExpressionMammalianPromoterCAGAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-rtTA
Plasmid#126034PurposePiggyBac cargo vector expressing rtTA. Also confers resistance to G418.DepositorInsertrtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
UsePiggybacExpressionMammalianPromoterhUbiCAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-mCherry
Plasmid#193355PurposeSelf-replicating RNA vector expressing mCherry fluorescent protein + PuroRDepositorInsertmCherry
UseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianPromoterT7Available SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Fyn FRET Biosensor
Plasmid#124654PurposeFRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPetDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
ExpressionMammalianAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-KRAB-dCas9-P2A-mCherry
Plasmid#60954Purpose2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherryDepositorInsertKRAB-dCas9-P2A-mCherry fusion
UseCRISPR and LentiviralTags2x NLS, HA, KRAB domain, and mCherryExpressionMammalianPromoterSFFVAvailable SinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-CFP
Plasmid#193357PurposeSelf-replicating RNA vector expressing CFP fluorescent protein + PuroRDepositorInsertCFP
UseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianPromoterT7Available SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ
Plasmid#64875Purposelentiviral expression of human POLQDepositorAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Klf5
Plasmid#50787Purposeretroviral expression of mouse Klf5DepositorAvailable SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0med
Plasmid#153286PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in neuronsDepositorHas ServiceAAV2InsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVPromoterhSynAvailable SinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_UBAP2L_mCherry
Plasmid#105286Purposepuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion proteinDepositorInsertUBAP2L (UBAP2L Human)
UseLentiviralTagsmCherryExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.RMacL3-01.EGFP.BCA5.WPRE
Plasmid#225685PurposeL3 pyramidal neuron Enhancer to drive transgene expressionDepositorInsertRMacL3-01.EGFP.BCA5
ExpressionMammalianAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.RMacL5ET-01.EGFP.BCA7.WPRE
Plasmid#225689PurposeL5 extratelencephalic projection neuron Enhancer to drive transgene expressionDepositorInsertRMacL5ET-01.EGFP.BCA7
ExpressionMammalianAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianPromoterCBh and U6Available SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only