We narrowed to 22,421 results for: Tes

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA1

Plasmid

#195827

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNA1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA1 (EBNA-1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SV40.eGFP.SV40(polyA)

Plasmid

#195558

Purpose

Expresses eGFP under control of short GFAP promoter

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

eGFP

Use

AAV

Tags

No tags

Expression

Mammalian

Promoter

short GFAP

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET41a iGluu

Plasmid

#119834

Purpose

Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluu

Tags

GST

Expression

Bacterial

Mutation

S72T

Promoter

T7

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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Puro-iNotch1IC

Plasmid

#75338

Purpose

doxycycline inducible Notch1 intracellular domain gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

Notch1 Intracellular domain (Notch1 Mouse)

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-SOX3

Plasmid

#61546

Purpose

Expresses SOX3 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
GFP-GLT1a

Plasmid

#162515

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

Plasmid

#216421

Purpose

Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Tags

3xmyc

Expression

Bacterial

Available Since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPbU6_hdhfr/yfcu

Plasmid

#216422

Purpose

Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Expression

Bacterial

Available Since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMW364

Plasmid

#238205

Purpose

Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pARF6(Q67L)-CFP

Plasmid

#11387

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pETM33_SUFU_Sufu

Plasmid

#178488

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SUFU_Sufu (SUFU Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_722

Plasmid

#216077

Purpose

CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral; Destination

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)

Plasmid

#199165

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTrex-Neo-tdTomato

Plasmid

#47975

Purpose

Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

tdTomato

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-FLEX-EGFP-mir30(Scn9a)

Plasmid

#79672

Purpose

Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP (Scn9a jellyfish, Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pKL001

Plasmid

#98917

Purpose

Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

CaPR

Expression

Bacterial

Promoter

BBa_J23118 - Strong Constitutive

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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REEP5-EGFP

Plasmid

#210363

Purpose

Expresses REEP5 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Tog

Plasmid

#29480

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K19R, E118G, G254R, K1834R

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP30_MARV

Plasmid

#135472

Purpose

Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

VP30

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-tdTomato-WPRE

Plasmid

#133786

Purpose

Cre-dependent tdTomato fluorescent reporter for cell labeling

Depositor

Michael Stryker

Article

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

pAAV-EF1a-DIO-tdTomato-WPRE

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt-DD

Plasmid

#39536

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (Akt1 Mouse)

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

DD = T308D and S473D

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP1

Plasmid

#125249

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMp1

Tags

NES signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pLKO-shPKM2_4

Plasmid

#42516

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

shRNA-PKM2 (PKM Human)

Use

Lentiviral

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 WT

Plasmid

#186467

Purpose

Expression of human MOSPD2 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TO-Vpr-mRuby3-IN

Plasmid

#167584

Purpose

For producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

Vpr-mRuby3-IN

Tags

Vpr-mRuby3

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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mKO3-TEAD4

Plasmid

#221217

Purpose

RFP fused TEAD4

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

mKO3-TEAD4 (TEAD4 )

Expression

Mammalian

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-1

Plasmid

#160701

Purpose

derivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

lacZ

Use

Synthetic Biology

Mutation

LacZ bordered by BsaI restrction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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