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174,739 results

Showing: 10601 - 10640 of 174739 results

  1. pNO286-3

    Plasmid
    #107746
    Purpose
    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)
    Depositor
    Jeffrey Tabor
    Article
    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb
    Inserts
    CcaS
    Ho1
    PcyA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Deleted AA 223 and 512
    Promoter
    J23106 and J23108
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107746-plasmid-map-sequence-id-209202

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  13. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97210-plasmid-map-sequence-id-182599

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  20. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98997-plasmid-map-sequence-id-189692

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  24. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92065-plasmid-map-sequence-id-181210

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  25. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  30. AICSDP-13:FBL-mEGFP

    Plasmid
    #87427
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    FBL Homology Arms with linker-mEGFP (FBL Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87427-plasmid-map-sequence-id-173980

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  34. LeGO-dKatushka2

    Plasmid
    #85214
    Purpose
    Lentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoter
    Depositor
    Boris Fehse
    Article
    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1
    Insert
    dKatushka2
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    BsrGI site removed at 5' end, BsrGI site ins…
    Promoter
    SFFV
    Available Since
    Jan. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    85214-plasmid-map-sequence-id-164348

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  40. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Expression
    Yeast
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

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Showing: 10601 - 10640 of 174739 results