173,219 results

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piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0323

Plasmid

#143394

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

LCORL (LCORL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNP3620 [pNP2916 - ISCro4 bRNA (TBL2, WT + DBL1, WT)]

Plasmid

#247267

Purpose

Express ISCro4 bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2916 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-mEKLF

Plasmid

#67833

Purpose

Expression of FL-EKLF driven by SV40 promoter

Depositor

James Bieker

Article

Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

EKLF (Klf1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pGolt-mCherry / pGolt3-mCherry

Plasmid

#73297

Purpose

Visualization of Dendritic Golgi Satellite

Depositor

Michael Kreutz

Article

Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

Inserts

ER export signal of Scap
ER export signal of Scap
TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)

Tags

ER export and mCherry

Expression

Mammalian

Promoter

CMV immediate early promoter

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

Plasmid

#108848

Purpose

Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

pMag,NLS

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ETV6_NTRK3

Plasmid

#205817

Purpose

Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ETV6_NTRK3 (ETV6, NTRK3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pXPG

Plasmid

#71248

Purpose

improved luciferase reporter gene plasmid

Depositor

Peter Cockerill

Article

Bert et al Plasmid. 2000 Sep;44(2):173-82.

Type

Empty backbone

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

none

Available Since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2b wt

Plasmid

#45447

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2B (Grin2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc. Inducible with tetracycline or doxycycline.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shControl-EGFP

Plasmid

#227685

Purpose

Expresses EGFP and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.18 CMV-Luc

Plasmid

#100984

Purpose

CMV luciferase reporter - firefly luciferase driven by CMV promoter

Depositor

Lee Helman

Article

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

CMV

Expression

Mammalian

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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synIRES_RC25

Plasmid

#189970

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

synIRES_RC25

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEF6.mCherry-TSG101

Plasmid

#38318

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

TSG101 (TSG101 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159816

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBUE411-B

Plasmid

#198234

Purpose

pPLTP::BBM cassette in pBUE411

Depositor

Andrea Gallavotti

Article

Chen et al bioRxiv 2022.09.02.506370

Insert

pPLTP::BBM cassette

Use

CRISPR; Plant binary vector

Expression

Plant

Promoter

PLTP

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Man1B1-GFP

Plasmid

#163646

Purpose

Expresses GFP tagged Man1B1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 1B member 1 (MAN1B1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smad3

Plasmid

#11742

Depositor

Jeff Wrana

Article

Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

Insert

SMAD3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_TauFRET_3xKQ_wpre

Plasmid

#207522

Purpose

Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

Depositor

Bradley Hyman

Article

Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

Insert

TauFRET_P301L_3xKQ

Use

Lentiviral

Tags

NeonGreen and mTurquoise2

Mutation

P301L; K311Q; K317Q; K321Q

Promoter

EF1a

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-V5His6_D

Plasmid

#146093

Purpose

Insect Expression of DmPABPC1

Depositor

Elisa Izaurralde

Insert

DmPABPC1 (pAbp Fly)

Expression

Insect

Mutation

one non-silent mutation G26S compared to the sequ…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLK

Plasmid

#201584

Purpose

Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p5193 pGL4-LCR-HPV16-LCR (nt 7000-100)-luciferase

Plasmid

#32888

Depositor

Peter Howley

Article

Ottinger et al Virology. 2009 Dec 20;395(2):161-71.

Insert

LCR HPV16

Promoter

LCR

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pnEA-vH_His-TEV-SEPT2_SEPT6

Plasmid

#174497

Purpose

bacterial co-expression of human SEPT2 and of human SEPT6

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

Plasmid

#138368

Purpose

Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-APEX1

Plasmid

#215126

Purpose

Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APEX1 (APEX1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1-mitoRexYFP

Plasmid

#60246

Purpose

Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

mitoRexYFP

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC(GD)/RagB(CTD) chimera

Plasmid

#112774

Purpose

express Flag-RagC (G domain) fused with with RagB (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain) fused with RagB (C terminal domain) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag RagB(GD)/RagC(CTD) chimera

Plasmid

#112773

Purpose

express Flag-RagB (Gdomain) fused with RagC (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-Laforin Myc R171H

Plasmid

#84671

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-Paxillin-22

Plasmid

#55114

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

Insert

Paxillin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-WASP

Plasmid

#242835

Purpose

Encodes human WASP and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

WASP (WAS Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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