161,531 results

1. pEBB HA RelA

   Plasmid

   #74892

   Purpose

   Express RelA in mammalian cells

   Depositor

   Colin Duckett, Ezra Burstein

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   RelA (RELA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR-Zeo

   Plasmid

   #158709

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Hsp60

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223-PRKAR1B

   Plasmid

   #23376

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PRKAR1B (PRKAR1B Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pci-EGFP-UPF1-DEAA

   Plasmid

   #135996

   Purpose

   DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   DE647AA

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-mTET2

   Plasmid

   #89735

   Purpose

   Expresses wildtype mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

   Plasmid

   #103866

   Purpose

   dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVH-EF1a-GFP-P2A-OCT4

   Plasmid

   #130692

   Purpose

   Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human OCT4 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Gs(short)-CASE

   Plasmid

   #168124

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG1

   Expression

   Mammalian

   Mutation

   NLuc is inserted at N136/V137 within GNAS

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP13786 - pAAV-AiE0140h-minBG-iCre(R297T)-BGHpA (Alias: CN3786)

   Plasmid

   #230382

   Purpose

   AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minRho

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUltra-H2B-mTagBFP2

    Plasmid

    #161791

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    EGFP and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6-CENP-A-mCherry

    Plasmid

    #89767

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CENP-A (CENPA Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrichoGate-16

    Plasmid

    #160716

    Purpose

    T-nos Terminator bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    nopaline synthase terminator (T-nos) bordered by BsaI restriction sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Incorporation of BsaI restriction sites

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ube2w

    Plasmid

    #31432

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2W Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pIRES2-mCherry-p53 deltaN

    Plasmid

    #49243

    Purpose

    expresses human p53 deltaN and mCherry in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 deltaN (TP53 Human)
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    deltaN ( lacks 39 residues at the N-terminus)

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. G13-CASE

    Plasmid

    #168127

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F125/D126 within GNA13

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. HSPA13_pCSdest

    Plasmid

    #53872

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    HSPA13 (HSPA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pLOX-CWBmi1

    Plasmid

    #12240

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter upstream of mBmi-1. LoxP in 3' …

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEos4b-Nup50-N-10

    Plasmid

    #57516

    Purpose

    Localization: Nuclear Pore, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Insert

    Nup50 (NUP50 Human)
    

    Use

    Tags

    mEos4b

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-mCherry-24xMBS

    Plasmid

    #86242

    Purpose

    Middle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    mCherry-24xMBS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTarget_CAST

    Plasmid

    #127926

    Purpose

    Target plasmid containing PSP1

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    PSP1 target (GGTT PAM)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TNIK gRNA (BRDN0001146045)

    Plasmid

    #75849

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TNIK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TNIK (TNIK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-SADB19L

    Plasmid

    #32632

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFB2C

    Plasmid

    #195285

    Purpose

    Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pC003 - LshC2C2 locus into pACYC184 for spacer cloning

    Plasmid

    #79152

    Purpose

    LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Use

    Tags

    Expression

    Mutation

    *

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBBR15.2-2H12.D11opt-scarRef

    Plasmid

    #221150

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2.1
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen2.1)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AARS_C1_pNIC-Bio3

    Plasmid

    #153046

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX6P1-hcGAS(157-522)

    Plasmid

    #108676

    Purpose

    For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

    Insert

    cGAS amino acid 157-522 (CGAS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acid 157-522

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MINK1 gRNA (BRDN0001146260)

    Plasmid

    #76260

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MINK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MINK1 (MINK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYL322d1/N-Vn

    Plasmid

    #183153

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AiP14478 - pAAV-AiE0456m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4478)

    Plasmid

    #229923

    Purpose

    AiE0456m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV.CMV.PI-si-HBV

    Plasmid

    #187859

    Purpose

    a human siRNA-expressing plasmid targeting HBV

    Depositor

    Yan Zeng

    Insert

    si-HBV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV R-CEPIA3mt

    Plasmid

    #140464

    Purpose

    Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

    Insert

    R-CEPIA3mt
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HCT46_rAAV-eHGT_1188m-minBglobin-SYFP2-WPRE3-BGHpA

    Plasmid

    #223943

    Purpose

    Direct-expressing SYFP2 AAV virus

    Depositor

    Tanya Daigle, The Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    minBglobin

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HCT49_rAAV-3Xcore-eHGT_1139m-minBglobin-SYFP2-WPRE3-BGHpA

    Plasmid

    #223944

    Purpose

    Direct-expressing SYFP2 AAV virus

    Depositor

    Tanya Daigle, The Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    minBglobin

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Arl13b-EGFP

    Plasmid

    #234844

    Purpose

    A mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Arl13b (ARL13B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GfaABC1D HRP-TM

    Plasmid

    #231499

    Purpose

    Expressing HRP on the cell surface of astrocytes

    Depositor

    Baljit Khakh

    Article

    Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

    Insert

    HRP
    

    Use

    AAV

    Tags

    Igk, HA, Myc, PDGFR-beta transmembrane domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-MCP-SRSF3-V5-mCherry

    Plasmid

    #235090

    Purpose

    SRSF3 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AiP12701: pAAV-hChATp-SYFP2-WPRE3-BGHpA (Alias: CN2701)

    Plasmid

    #236601

    Purpose

    hChATp is a promoter fragment with activity in striatal cholinergic neurons

    Depositor

    Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge