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161,273 results

Showing: 10681 - 10720 of 161273 results

  3. AICSDP-23:TJP1-mEGFP

    Plasmid
    #87429
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87429-plasmid-map-sequence-id-174170

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  9. qTAG-AAVS1-PGK-Blast

    Plasmid
    #227270
    Purpose
    Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227270-plasmid-map-sequence-id-452024

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  18. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

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  19. mCherry-ATG5-C-18

    Plasmid
    #54995
    Purpose
    Localization: Membrane Receptor, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ATG5 (ATG5 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Use
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  27. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  28. sfGFP-Caveolin-C-10

    Plasmid
    #56366
    Purpose
    Localization: Membrane, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Caveolin (CAV1 Human)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. ZsGreen1-N1

    Plasmid
    #54702
    Purpose
    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ZsGreen1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54702-plasmid-map-sequence-id-266653

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  31. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

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  32. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid
    #100834
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    CamKII
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    100834-plasmid-map-sequence-id-259370

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  33. pCAG-VSVM

    Plasmid
    #64086
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene
    Depositor
    Ian Wickersham
    Insert
    VSV matrix protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64086-plasmid-map-sequence-id-107218

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  37. pCytERM-mhYFP

    Plasmid
    #186530
    Purpose
    Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Monomeric hyperfolder YFP
    Use
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186530-plasmid-map-sequence-id-368240

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Showing: 10681 - 10720 of 161273 results