We narrowed to 22,837 results for: Sis

1. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK 3xFLAG-TWIST2 neo

   Plasmid

   #197936

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. YFP-Rac1(L61)

   Plasmid

   #11401

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Glutamine 61 mutated to Leucine.

   Available Since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

4. FUW-M2-PAK2 wt

   Plasmid

   #31680

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   p21-activated protein kinase 2 (PAK2 Human)
   

   Use

   Lentiviral

   Tags

   M2

   Expression

   Mammalian

   Available Since

   April 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. optoPKAW196R/Y204A

   Plasmid

   #104546

   Purpose

   Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Cry2-mCherry-PKA W196R/Y204A
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   PKA W196R and Y204A

   Promoter

   CMV

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB423

   Plasmid

   #74817

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-mNINJ1-D53A-GFP

   Plasmid

   #208778

   Purpose

   MSCV vector expressing D53A mouse NINJ1

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   Changed aspartic acid 53 to alanine (D53A)

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ macroH2A1.2-GFP-HA

   Plasmid

   #30515

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

   Insert

   macroH2A1.2-C (Macroh2a1 Mouse)
   

   Use

   Xenopus/zebrafish expression.

   Tags

   eGFP and triple HA

   Expression

   Mammalian

   Mutation

   NO STOP

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T1-Rac1 G15A

   Plasmid

   #69355

   Purpose

   expression of nucleotide free Rac1 in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G15A

   Promoter

   tac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pHH0103_SNX18-1/1

    Plasmid

    #91369

    Purpose

    Protein expression and purification of human SH3 domain construct SNX18-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SNX18-1/1 (SNX18 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD2KN0908

    Plasmid

    #177088

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    P19, CrDXS2, PaGPPS1, and CrGES
    

    Use

    Synthetic Biology

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWL89A mmPing20

    Plasmid

    #145788

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20
    

    Expression

    Yeast

    Mutation

    T162C, T258A, T287C, T300A, T304A, T310A, G372A

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVXPuro-epsilon-COP-GFP

    Plasmid

    #66604

    Purpose

    Expresses epsilon COP as a GFP fusion

    Depositor

    David Stephens

    Article

    Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

    Insert

    epsilon COP (COPE Bovine)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pJMP1223

    Plasmid

    #119261

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1221

    Plasmid

    #119260

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSicoR human Dicer2

    Plasmid

    #14764

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Dicer shRNA (DICER1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. YFP-PCAF-C2

    Plasmid

    #134572

    Purpose

    Expresses YFP-PCAF fusion protein

    Depositor

    Susan Janicki

    Article

    Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

    Insert

    PCAF (Kat2b Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23a-HsUBB

    Plasmid

    #69562

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (UBB Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pCAGGS-Flag-hsDicer (K70A)

    Plasmid

    #41589

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K70A

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

22. FKBP_cSbMVp

    Plasmid

    #118968

    Purpose

    Expresses C-terminus of soybean mosaic virus protease (SbMVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cSbMVp in fusion with FKBP
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA5/FRT/TO DNAJB4

    Plasmid

    #19472

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCK004 ubi:mCerulean_SV40 (#302)

    Plasmid

    #195978

    Purpose

    Expression construct

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubi:mCerulean
    

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. cycLuc_PPVs

    Plasmid

    #119208

    Purpose

    Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with PPVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Promoter

    CMV

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJMP1335

    Plasmid

    #119269

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 3537 pMIG Bim

    Plasmid

    #8786

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bim (Bcl2l11 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pSicoR human Drosha2

    Plasmid

    #14767

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Drosha shRNA (DROSHA Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

29. p6895 PHAGE-P CMVT N-HA-hE6AP I wt

    Plasmid

    #37601

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I wt (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV tet regulatable

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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30. H2B-mScarlet- hRec8(297-506)-mNeonGreen

    Plasmid

    #174717

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Rec8

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Rec8 (REC8 Human)
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    297-506

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSuper mouse DGCR8-1

    Plasmid

    #14772

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    DGCR8 shRNA (Dgcr8 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

32. pET-NtLAtC60αβC20

    Plasmid

    #160873

    Purpose

    Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: pBAD, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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33. pDest Lenti CA-GFP

    Plasmid

    #197459

    Purpose

    Bicistronic expression vector expressing CA-GFP under the CMV promotor separated by an internal ribosome entry site (IRES) mediated expression of dTomato reporter protein.

    Depositor

    Omar Khan

    Article

    Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S65T

    Promoter

    CMV, IRES

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDGE670

    Plasmid

    #153236

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA6 MCV LTco S239A

    Plasmid

    #112194

    Purpose

    Expression of MCPyV LT mutant (Ser 239 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. peCALWY-3

    Plasmid

    #22235

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-3 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    Similar to eCALWY-1, but with a linkerlength of 3…

    Available Since

    Nov. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pBABE-puro-mIR-31 sponge

    Plasmid

    #22694

    Purpose

    Stable suppression of miR-31 function

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Tags

    *

    Expression

    Mammalian

    Mutation

    miR-31 sponge with 7x tandem binding sites to the…

    Available Since

    March 11, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid

    #22931

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaC (PHLPP1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PHLPP-deltaC, deletion of PDZ binding domain--the…

    Promoter

    CMV

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

39. pCW57.1-ZNF746

    Plasmid

    #207825

    Purpose

    Dox-inducible expression of ZNF746

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746 (ZNF746 Human)
    

    Use

    Lentiviral

    Promoter

    TRE

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDGE463

    Plasmid

    #153234

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge