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pcDXC3GMS

Plasmid

#49031

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0134

Plasmid

#162307

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, HiBiT-stop, frame1 (NNT-TCG)

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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RAD Module G8-C1

Plasmid

#38208

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

SET Integrase
Bxb1 Excisonase
RESET Integrase

Use

Synthetic Biology; Biobrick cloning vector

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

Plasmid

#164263

Purpose

Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Type

Empty backbone

Expression

Bacterial

Promoter

Arabinose

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA4c-RFP

Plasmid

#35299

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pPTK020-7-attB

Plasmid

#84989

Purpose

Type 7, BxbI recognition site

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

BxbI recognition site

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1711 t to a)

Promoter

Non

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK006-3a-αMF no EAEA

Plasmid

#84965

Purpose

Type 3a, α-mating factor no EAEA - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor no EAEA secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGL0_22 [pSG5 ORI]

Plasmid

#198935

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJL005

Plasmid

#92327

Purpose

Express FtsZ tagged with mEos3.2 in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBW213ara-hrpS

Plasmid

#61435

Purpose

pBAD18-cm encoding rbs33-hrpS-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbs33-hrpS-ter

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pNH034

Plasmid

#42150

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

RT-cassette

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCJH059_proD_Cas12c_DualRNA-GFP_CAM

Plasmid

#183076

Purpose

Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and pre-crRNA

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS-Flag

Plasmid

#17364

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2003 Feb . 77(3):1820-9.

Insert

MMLV proviral DNA

Tags

Flag

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGT-Ah2

Plasmid

#122569

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGL0_19 [panARS ORI]

Plasmid

#198932

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

panARS ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-CC

Plasmid

#105117

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mVenus/tagRFP

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFF706

Plasmid

#61448

Purpose

Light-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pDawn

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFF714

Plasmid

#61449

Purpose

IPTG-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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