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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2GFP

Plasmid

#82416

Purpose

3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

GFP

Use

CRISPR and Lentiviral

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE2max-∆RNaseH-dualU6

Plasmid

#198735

Purpose

AAV genome encoding C-terminal PE2max and U6 expression cassettes

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

NpuC-CtermPE2max∆RNaseH

Use

AAV

Promoter

Cbh

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Article

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pH-ABE8e

Plasmid

#200774

Purpose

For plant adenine base editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

Insert

nCas9(D10A)-TadA8e

Use

CRISPR

Expression

Plant

Mutation

D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

Promoter

maize Ubiquitin-1, OsU3

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHS2056

Plasmid

#239837

Purpose

OMEGAOff + omegaRNA scaffold AAV expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold

Use

AAV

Tags

HA and SV40 NLS

Mutation

Insertion of REC domain from NbaCas9-1 + E137K/E4…

Promoter

EFS; human U6

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-YE1-BE4max

Plasmid

#198554

Purpose

Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

Plasmid

#138148

Purpose

Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NES

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pB-rtTA

Plasmid

#126034

Purpose

PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Insert

rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735

Use

Piggybac

Expression

Mammalian

Promoter

hUbiC

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-EF1α-mKate2-biPAC

Plasmid

#169127

Purpose

AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

Depositor

Georg Nagel

Article

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Insert

mKate2-biPAC

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

codon optimized to mouse

Promoter

EF1a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.2-T2A-EGFP

Plasmid

#216490

Purpose

Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2FE-ABE8e-SpRY

Plasmid

#213008

Purpose

A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

Depositor

Richard Sherwood

Article

Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

Insert

ABE8e-SpRY-D10A

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A nickase variant of SpRY

Promoter

Ef-1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

Plasmid

#216494

Purpose

Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAktKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-T2A-EGFP

Plasmid

#216489

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

Plasmid

#199604

Purpose

Expression of cell permeable spCas9 in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

Cas9-T6N

Use

CRISPR

Expression

Bacterial

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-YE1-BE4max

Plasmid

#198546

Purpose

Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Control-Control-LRG-GFP

Plasmid

#225873

Purpose

Two copies of guide RNA targeting a control sequence

Depositor

Janai Carr-Ascher

Article

Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

Insert

Control

Use

CRISPR and Lentiviral

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

Plasmid

#199605

Purpose

Expression of cell permeable opAsCas12a in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

opCas12a-T8N

Use

CRISPR

Tags

6xHis, Twin-strep, SUMO

Expression

Bacterial

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER dCas9-TET1CD

Plasmid

#101921

Purpose

The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

dCas9-TET1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEf1a-goldDn29-P2A-GFP

Plasmid

#247153

Purpose

Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-Bcl-XL

Plasmid

#46972

Purpose

lentiviral expression of Bcl-XL

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ezh2FL

Plasmid

#235575

Purpose

Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ezh2 (Ezh2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-G9aCD

Plasmid

#235580

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

G9a (Ehmt2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBD-PYR1_star-MANDI

Plasmid

#241280

Purpose

Yeast two hybrid vector expressing BD-PYR1_star-MANDI

Depositor

Sean Cutler

Article

Park et al Nat Chem Biol. 2024 Jan;20(1):103-110. d

Insert

PYR1_star_mandi (PYR1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

Plasmid

#222852

Purpose

Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Dot1LCD

Plasmid

#235582

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dot1l (Dot1l Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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