19,654 results

1. pHUE411

   Plasmid

   #62203

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   OsU3 and Ubi

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCMV-intron myc Rab2WT

   Plasmid

   #46779

   Purpose

   expression of WT Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pHIV-Luciferase

   Plasmid

   #21375

   Purpose

   self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

   Depositor

   Bryan Welm

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

   Plasmid

   #61628

   Purpose

   Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   ntdTomato
   n2xEGFP
   

   Expression

   Plant

   Mutation

   2 copies of EGFP with N-terminal SV40 NLS

   Promoter

   Auxin-dependent DR5 promoter containing 9 reverse…

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. NES-EGFP-PH-ARNO2G-I303Ex2

   Plasmid

   #116868

   Purpose

   PIP3 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   CYTH2 (CYTH2 Human)
   

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amind acids 252-399, isoleucine 303 changed to gl…

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc-GFPC1-Sec61beta

   Plasmid

   #15108

   Depositor

   Tom Rapoport

   Insert

   Sec61beta (SEC61B Human)
   

   Tags

   Ac GFP

   Expression

   Mammalian

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. GW1CMV-Perceval

   Plasmid

   #21737

   Purpose

   can be used to monitor the ATP:ADP ratio during live-cell imaging

   Depositor

   Gary Yellen

   Article

   Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

   Insert

   Perceval (fluorescent ATP:ADP sensor)
   

   Tags

   5'UTR

   Expression

   Mammalian

   Mutation

   improved Kozak sequence

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-cytoEKAR4

   Plasmid

   #174438

   Purpose

   Cytosol-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   cytoEKAR4
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. USP2

   Plasmid

   #36894

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP2 (USP2 Human)
   

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Available Since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. EGFP-E-Syt3

    Plasmid

    #66832

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt3 (ESYT3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1-GCaMP6m-Xn

    Plasmid

    #118976

    Purpose

    Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

    Depositor

    Xiaodong Liu

    Article

    Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

    Insert

    GCaMP6m-XN
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.3_OCT4

    Plasmid

    #26816

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-OCT4-3'UTR (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLenti6/V5-DEST-RNF2

    Plasmid

    #31216

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    RNF2 (RNF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pZT-AAVS1-R1

    Plasmid

    #52638

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Right AAVS1 TALEN
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-TLR8-YFP

    Plasmid

    #13024

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 8 (TLR8 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mNeptune2.5

    Plasmid

    #51310

    Purpose

    Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2.5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-human Igf2bp2-IRES-GFP

    Plasmid

    #91890

    Purpose

    Retroviral expression of human Igfbp2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Igf2bp2 (IGF2BP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEP4 E02S CK2M EN2L

    Plasmid

    #20924

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. p75-RFP

    Plasmid

    #24092

    Depositor

    Moses Chao

    Insert

    p75NTR (NGFR Human)
    

    Tags

    DSRed

    Expression

    Mammalian

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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